Decay kinetics of HIV-1 specific T cell responses in vertically HIV-1 exposed seronegative infants.

Frontiers in Immunology
Sara J HolditchDouglas F Nixon

Abstract

The majority of infants born, in developed countries, to HIV-1 positive women are exposed to the HIV-1 virus in utero or peri/post-partum, but are born uninfected. We, and others, have previously shown HIV-1 specific T cell responses in HIV-1 exposed seronegative (HESN) neonates/infants. Our objective in this study was to examine the rate of decay in their HIV-1 specific T cell response over time from birth. Cross-sectional and longitudinal studies of HIV-1 specific T cell responses in HESN infants were performed. Peripheral blood mononuclear cells (PBMC) were isolated from 18 HIV-1 DNA PCR negative infants born to HIV-1 infected mothers receiving care at the Jacobi Medical Center, Bronx, NY, USA. PBMC were examined for T cell responses to HIV-1 antigens by interferon-gamma (IFN-γ) ELISPOT. PBMC from 15 HESN neonates/infants were analyzed. We observed a decay of HIV-1 specific T cell responses from birth at a rate of -0.599 spot forming unit/10⁶ cells per day, with a median half-life decay rate of 21.38 weeks (13.39-115.8). Our results support the dynamic nature of T cell immunity in the context of a developing immune system. The disparate rate of decay with studies of adults placed on antiretroviral drugs suggests that antigen...Continue Reading

Citations

Nov 5, 2019·Journal of Perinatal Medicine·Clara L GasthausMareike Möllers
Aug 20, 2019·Scientific Reports·Benjamin GabrielBarbara L Lohman-Payne

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Methods Mentioned

BETA
density gradient centrifugation
PCR
PCRs

Software Mentioned

Prism
GraphPad

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