Decellularization of porcine skeletal muscle extracellular matrix for the formulation of a matrix hydrogel: a preliminary study

Journal of Cellular and Molecular Medicine
Yuehe FuQing Lv

Abstract

Extracellular matrix (ECM) hydrogels are used as scaffolds to facilitate the repair and reconstruction of tissues. This study aimed to optimize the decellularization process of porcine skeletal muscle ECM and to formulate a matrix hydrogel scaffold. Five multi-step methods (methods A-E) were used to generate acellular ECM from porcine skeletal muscle [rinsing in SDS, trypsin, ethylenediaminetetraacetic acid (EDTA), Triton X-100 and/or sodium deoxycholate at 4-37°C]. The resulting ECM was evaluated using haematoxylin and eosin, 4-6-diamidino-2-phenylindole (DAPI) staining, and DNA quantification. Acellular matrix was dissolved in pepsin and gelled at 37°C. Hydrogel response to temperature was observed in vivo and in vitro. ECM components were assessed by Masson, Sirius red, and alcian blue staining, and total protein content. Acellular porcine skeletal muscle exhibited a uniform translucent white appearance. No intact nuclear residue was detected by haematoxylin and eosin staining, while DAPI staining showed a few nuclei in the matrixes produced by methods B, C, and D. Method A generated a gel that was too thin for gelation. However, the matrix obtained by rinsing in 0.2% trypsin/0.1% EDTA, 0.5% Triton X-100, and 1% Triton X-100...Continue Reading

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Citations

Oct 31, 2017·International Journal of Molecular Sciences·Nor Kamalia ZahariShiplu Roy Chowdhury
Apr 1, 2018·Veterinary Surgery : VS·Tiffany L SarrafianSusan M Stover
Oct 9, 2018·Biotechnology Progress·Rajesh Guruswamy Damodaran, Patrick Vermette
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Jun 18, 2020·Frontiers in Bioengineering and Biotechnology·Gabriel Romero LiguoriMartin Conrad Harmsen
Jul 28, 2020·Frontiers in Bioengineering and Biotechnology·Nicola Contessi NegriniLina Altomare
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Jul 3, 2021·International Journal of Molecular Sciences·Fernando Leiva-CepasJose Peña-Amaro
Aug 29, 2021·Differentiation; Research in Biological Diversity·Mohammadreza SafdariJavad Hashemi

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Methods Mentioned

BETA
light microscopy
Assay
scanning electron microscopy
DNA Assay

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