Decellularized caprine liver-derived biomimetic and pro-angiogenic scaffolds for liver tissue engineering

Materials Science & Engineering. C, Materials for Biological Applications
Tarun AgarwalSudip Kumar Ghosh

Abstract

The development of a pre-vascularized liver tissue construct with native-like 3-dimensional (3D) microarchitecture and extracellular matrix (ECM) composition is essential to meet the current demand for liver transplantation. Here, we report the methodology and in-depth characterization of the decellularized caprine liver scaffold (CLECM-S) for tissue engineering application. CLECM-S retained crucial ECM components and structural features similar to the native liver tissue. For comparative evaluation, conventional glutaraldehyde crosslinked collagen scaffold (Col-S) was taken as a control. CLECM-S underwent a slow but sustained swelling and collagenase mediated degradation and had the mechanical stiffness closer to that of the native human liver. HepG2 cells cultured on CLECM-S exhibited an enhanced expression of mature and functional hepatocyte markers. In addition, CLECM-S also showed pro-angiogenic properties as confirmed by Chick Chorioallantoic Membrane (CAM) assay. Upon implantation in a mouse model, the scaffolds did not elicit any significant immunogenic response. These results, together, provide a solid evidence depicting superiority of CLECM-S over conventional Col-S for liver tissue engineering applications.

Citations

Jun 25, 2020·Journal of Biomaterials Applications·Di MengGuifeng Zhang
Oct 19, 2020·Stem Cell Reviews and Reports·Akram NadiShiva Asadpour
Nov 4, 2020·Tissue Engineering. Part B, Reviews·Quanfu XuShengjie Ling
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Tarun AgarwalIbrahim T Ozbolat
Apr 20, 2021·Advanced Healthcare Materials·Chao Tao, Dong-An Wang
Nov 19, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Hengameh BakhtiarAnil Kishen
Aug 3, 2019·International Journal of Biological Macromolecules·Tarun AgarwalManchikanti Padmavati
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Maria Stefania MassaroVladimíra Moulisová
Jul 14, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hemant SinghNarayan Chandra Mishra
Sep 9, 2019·ACS Biomaterials Science & Engineering·Tarun AgarwalTapas Kumar Maiti
Oct 12, 2020·Cells, Tissues, Organs·Lanuza Alaby Pinheiro FaccioliRegina Coeli Dos Santos Goldenberg

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