Decellularized liver transplant could be recellularized in rat partial hepatectomy model

Journal of Biomedical Materials Research. Part a
Erfani M NaeemVahid Razban

Abstract

In situ recellularization of the liver decellularized scaffold is a potential therapeutic alternative for liver transplantation. We aimed to develop an in situ procedure for recellularization of the rat liver using sodium lauryl ether sulfate (SLES) compared with Triton X-100/SDS. Rat liver specimens were rinsed with PBS, decellularized with either Triton X-100/SDS or SLES, and finally rinsed by distilled water. The efficiency of decellularized liver scaffolds was evaluated by histological, confocal Raman microscopy, histochemical staining, and DNA quantification assessments. Finally, in vivo studies were done to assess the biocompatibility of the liver scaffold by serum biochemical parameters and the recellularization capacity by histological and immunohistochemistry staining. Findings confirmed the preservation of extracellular matrix (ECM) components such as reticular, collagen, glycosaminoglycans, and neutral carbohydrates in both Triton X-100/SDS- and SLES-treated livers. Hoechst, feulgen, Hematoxylin and eosin, and DNA quantification assessments confirmed complete genetic content removal. The serological parameters showed no adverse impact on the liver functions. Transplantation of SLES-treated cell-free decellularized li...Continue Reading

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Citations

Mar 18, 2020·Journal of Biomedical Materials Research. Part a·Tarek SalehHeung-Myong Woo
Jul 7, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Asrin EmamiNehleh Zarei Fard
Aug 18, 2020·Organogenesis·Marlon L DiasRegina C S Goldenberg
Nov 23, 2019·Journal of Translational Medicine·Vahid HosseiniReza Rahbarghazi
Aug 28, 2021·Systems Biology in Reproductive Medicine·Sanaz AlaeeTahereh Talaei-Khozani

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