Decellularized xenogenic cartilage extracellular matrix (ECM) scaffolds for the reconstruction of osteochondral defects in rabbits.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Piyali DasSamit Kumar Nandi

Abstract

The use of decellularized native allogenic or xenogenic cartilaginous extracellular matrix (ECM) biomaterials is widely expanding in the fields of tissue engineering and regenerative medicine. In this study, we aimed to develop an acellular, affordable, biodegradable, easily available goat conchal cartilaginous ECM derived scaffolding biomaterial for repair and regeneration of osteochondral defects in rabbits. Cartilages harvested from freshly collected goat ears were decellularized using chemical agents, namely, hypotonic-hypertonic (HH) buffer and Triton X-100 solution, separately. The morphologies and ultrastructure orientations of the decellularized cartilages remained unaltered in spite of complete cellular loss. Furthermore, when the acellular cartilaginous ECMs were cultured with murine mesenchymal stem cells (MSCs) (C3H10T1/2 cells), cellular infiltration and proliferation were thoroughly monitored using SEM, DAPI and FDA stained images, whereas the MTT assay proved the biocompatibility of the matrices. The increasing amounts of secreted ECM proteins (collagen and sGAG) indicated successful chondrogenic differentiation of the MSCs in the presence of the treated cartilage samples. In vivo biocompatibility studies showed ...Continue Reading

References

Aug 1, 1987·Journal of Biomedical Materials Research·R SmithD F Williams
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C W ChenW F Lai
Dec 14, 2005·Current Opinion in Rheumatology·Catherine K KuoRocky S Tuan
Aug 24, 2007·Osteoarthritis and Cartilage·Y M Bastiaansen-JenniskensJ Degroot
Sep 28, 2007·Biomaterials·Christoph ErggeletChristian Kaps
Jan 24, 2009·Journal of Biomedical Materials Research. Part a·Chet C XuKaren S Pawlowski
Mar 31, 2009·Trends in Biotechnology·Claire VinatierDanièle Noël
Jan 1, 2009·Critical Reviews in Biomedical Engineering·Lijie ZhangKyriacos A Athanasiou
Apr 23, 2010·Tissue Engineering. Part a·Joaquin CortiellaJoan E Nichols
Feb 8, 2011·Biomaterials·Peter M CrapoStephen F Badylak
Jul 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Avital MendelsonJeremy J Mao
Aug 23, 2011·Journal of Biomedical Materials Research. Part a·Ehab KheirEileen Ingham
Jun 14, 2012·Tissue Engineering. Part a·Silke SchwarzNicole Rotter
Jan 10, 2013·Trends in Biotechnology·Kim E M BendersJos Malda
Mar 19, 2013·International Journal of Pharmaceutics·Eva FilováEvžen Amler
Jul 3, 2013·Tissue Engineering. Part B, Reviews·Henning MadryMagali Cucchiarini
Oct 23, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Hana HrebikovaJaroslav Mokry
Mar 29, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Weiguang WangKaren M Lyons
Sep 13, 2014·Annals of Biomedical Engineering·Ricardo Londono, Stephen F Badylak
Dec 23, 2014·Biomaterials Science·Lindsay E Fitzpatrick, Todd C McDevitt
Nov 8, 2016·Acta Biomaterialia·Kathryne S BrockmanJ Paul Santerre
Feb 15, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marek KaweckiAleksandra Kasperczyk
Mar 27, 2018·Journal of Biomedical Materials Research. Part a·Steve ElderAvery Cooley
Oct 26, 2018·Journal of Tissue Engineering and Regenerative Medicine·Rupnarayan BhattacharyaSamit Kumar Nandi
Mar 31, 2020·Methods in Cell Biology·Fátima Raquel MaiaJoaquim Miguel Oliveira
Sep 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yogendra Pratap SinghBiman B Mandal
Apr 27, 2020·Methods in Cell Biology·Piyali DasSamit Kumar Nandi

❮ Previous
Next ❯

Software Mentioned

VGStudio MAX

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.