Decidual-secreted factors alter invasive trophoblast membrane and secreted proteins implying a role for decidual cell regulation of placentation.

PloS One
E MenkhorstEvdokia Dimitriadis

Abstract

Inadequate or inappropriate implantation and placentation during the establishment of human pregnancy is thought to lead to first trimester miscarriage, placental insufficiency and other obstetric complications. To create the placental blood supply, specialized cells, the 'extravillous trophoblast' (EVT) invade through the differentiated uterine endometrium (the decidua) to engraft and remodel uterine spiral arteries. We hypothesized that decidual factors would regulate EVT function by altering the production of EVT membrane and secreted factors. We used a proteomics approach to identify EVT membrane and secreted proteins regulated by decidual cell factors. Human endometrial stromal cells were decidualized in vitro by treatment with estradiol (10(-8) M), medroxyprogesterone acetate (10(-7) M) and cAMP (0.5 mM) for 14 days. Conditioned media (CM) was collected on day 2 (non-decidualized CM) and 14 (decidualized CM) of treatment. Isolated primary EVT cultured on Matrigel™ were treated with media control, non-decidualized or decidualized CM for 16 h. EVT CM was fractionated for proteins <30 kDa using size-exclusion affinity nanoparticles (SEAN) before trypsin digestion and HPLC-MS/MS. 43 proteins produced by EVT were identified; 1...Continue Reading

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Citations

Dec 4, 2014·Clinical Proteomics·Sonia BaigMahesh Choolani
Jan 13, 2016·American Journal of Reproductive Immunology : AJRI·Shipra SharmaDeepak Modi
Aug 29, 2014·Reproduction, Fertility, and Development·E MenkhorstE Dimitriadis
Feb 22, 2013·Molecular Reproduction and Development·Gael L M Cagnone, Marc-André Sirard
Sep 16, 2016·Journal of Reproductive Immunology·V SalleP Duhaut
Aug 31, 2019·Cell Biology International·Thádia Evelyn de AraújoIdessania Nazareth da Costa
Jul 15, 2020·Biophysics Reviews·Rhonda J Davey, Pierre Dj Moens
Aug 31, 2020·Hypertension·Ellen MenkhorstEva Dimitriadis
Jun 12, 2020·Integrative and Comparative Biology·Daniel J Stadtmauer, Günter P Wagner
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Eliza GrbacEllen Menkhorst
Sep 2, 2021·Journal of Gynecology Obstetrics and Human Reproduction·Habibe AyvacıOya Demirci
Aug 20, 2017·Scientific Reports·E M MenkhorstE Dimitriadis

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Methods Mentioned

BETA
biopsies
ELISA

Software Mentioned

Adobe Photoshop
mascot

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