Decidualization modulates a signal transduction system via protease-activated receptor-1 in endometrial stromal cells

American Journal of Reproductive Immunology : AJRI
Kaori GotoH Narahara

Abstract

Decidual cells are thought to be involved in the maintenance of pregnancy. We conducted this study to evaluate the cellular function of endometrial stromal cells (ESCs) transitioning to decidualization. Normal endometrial specimens were obtained from premenopausal patients who had undergone hysterectomies for subserosal leiomyomas. Decidualization of the ESCs (DSCs) was induced by incubating subconfluent cells in media containing medroxyprogesterone acetate and dibutyryl-cyclic adenosine monophosphate. We first analyzed the expression profile of protease-activated receptor-1 (PAR-1) between ESCs and DSCs. To investigate the intracellular signal transduction system in the DSCs, we incubated cells with thrombin receptor activator peptide 6 (TRAP-6). The levels of IL-8, monocyte chemo-attractant protein-1, matrix metalloproteinase (MMP)-1, and vascular endothelial growth factor in the culture medium were measured by enzyme-linked immunosorbent assays. The activation of the MAP kinase signaling pathway was detected by a Western blot analysis. The activation was evaluated for the expression of p21. PAR-1 receptor expression is upregulated in DSCs. The productions of chemokine and MMP-1 increased in the DSCs with the addition of TRAP...Continue Reading

References

Aug 19, 1991·FEBS Letters·U B RasmussenE Van Obberghen-Schilling
Oct 17, 1991·Nature·T K VuS R Coughlin
Jul 1, 1980·The Journal of Clinical Endocrinology and Metabolism·I A MaslarD H Riddick
Jun 1, 1994·Human Reproduction·R G Edwards
Jun 11, 2002·Biochemical and Biophysical Research Communications·Jason A Lehman, Julian Gomez-Cambronero
Sep 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Charles J LockwoodFrederick Schatz
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Hong WangGeorg Reiser
Sep 12, 2003·The Journal of Endocrinology·B Gellersen, J Brosens
Jan 30, 2004·American Journal of Obstetrics and Gynecology·Grozdana BilicRoland Zimmermann
Mar 10, 2005·The Journal of Clinical Endocrinology and Metabolism·Harunobu MatsumotoIsao Miyakawa
Mar 14, 2007·Molecular Human Reproduction·J Julie KimA T Fazleabas
Oct 26, 2007·Seminars in Reproductive Medicine·Birgit GellersenJan J Brosens
Dec 16, 2010·Human Reproduction·Yasushi KawanoHisashi Narahara
Mar 1, 2012·American Journal of Reproductive Immunology : AJRI·Akitoshi NakashimaShigeru Saito
Jan 10, 2018·Advances in Pharmacology·Xi Wang, Raouf A Khalil

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.