Decidual/trophoblast prolactin-related protein: characterization of gene structure and cell-specific expression

Endocrinology
K E OrwigM J Soares

Abstract

Decidual/trophoblast PRL-related protein (d/tPRP) is a member of the PRL gene family and is dually expressed in uterine and placental tissues in a highly coordinated pattern during pregnancy. In the present study, we describe the isolation and characterization of the d/tPRP gene. A lambda DASH II Wistar-Kyoto rat genomic library was screened with a labeled d/tPRP complementary DNA, resulting in the isolation of two phage clones, RGLd-41 [17.7 kilobases (kb)] and RGLd-42 (15.8 kb). RGLd-41 alone was found to contain the full-length d/tPRP gene and was used for subsequent analyses. The d/tPRP gene possesses a six-exon, five-intron organization. Relative to other highly conserved members of the PRL gene family, d/tPRP contains a single small additional exon (exon 3) situated between exons 2 and 3 of the prototypical PRL gene. The region corresponding to exon 3 of d/tPRP encodes for a unique amino acid region found in a subset of PRL family members. A reverse transcription-PCR (RT-PCR) tissue survey for d/tPRP messenger RNA revealed that d/tPRP expression was restricted to decidual and trophoblast tissues. A single transcription start site 65 bp upstream of the initiation codon was identified in decidual tissue, whereas multiple tr...Continue Reading

Citations

Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·T NozakiT Sugimura
Apr 6, 2007·Molecular Reproduction and Development·Daisuke KomatsuKatsuhiko Takahashi
Aug 26, 2015·PLoS Genetics·Juanmahel DavilaIndrani C Bagchi
Aug 26, 1998·Biology of Reproduction·V RiderW M Justice
Dec 19, 2002·Endocrinology·Dustin O WiemersMichael J Soares
Feb 5, 2015·American Journal of Physiology. Endocrinology and Metabolism·Keishiro IsayamaMasa-aki Hattori
May 20, 2008·Archives of Physiology and Biochemistry·Yang YuReinald Fundele
Nov 7, 2017·Birth Defects Research·Michael J SoaresKeisuke Kozai
Dec 23, 1999·The Journal of Biological Chemistry·A Prigent-TessierG Gibori

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