Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing

Cell Reports
Isabelle StévantSerge Nef


The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during sex determination, we identified a single population of somatic progenitor cells prior to sex determination. A subset of these progenitors differentiates into Sertoli cells, a process characterized by a highly dynamic genetic program consisting of sequential waves of gene expression. Another subset of multipotent cells maintains their progenitor state but undergoes significant transcriptional changes restricting their competence toward a steroidogenic fate required for the differentiation of fetal Leydig cells. Our findings confirm the presence of a unique multipotent progenitor population in the gonadal primordium that gives rise to both supporting and interstitial lineages. These also provide the most granular analysis of the transcriptional events occurring during testicular cell-fate commitment.


May 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Abigail HarrisAndy Greenfield
May 18, 2018·Endocrine Reviews·Emmi RotgersHumphrey Hung-Chang Yao
Jul 26, 2018·Journal of Molecular Endocrinology·Yisheng YangMegan Wilson
Nov 2, 2019·Genes, Brain, and Behavior·Nicole Leitner, Yehuda Ben-Shahar
Apr 11, 2020·Endocrinology·Penny A F WhileyKate L Loveland
Aug 1, 2020·Molecular Reproduction and Development·Helena D Zomer, Prabhakara P Reddi
Jun 9, 2020·Human Molecular Genetics·Yasmin TatourTamar Ben-Yosef
Oct 31, 2018·Nature Communications·Deepti L Kumar, Tony DeFalco
Oct 15, 2019·Reproductive Medicine and Biology·Yuichi Shima
Nov 9, 2018·Frontiers in Genetics·Daniel Rodríguez GutiérrezAnna Biason-Lauber
Dec 15, 2019·Genetics·Catherine LivermoreAndy Greenfield
May 16, 2020·Human Reproduction·Estelle LecluzeFrédéric Chalmel
Dec 7, 2018·Development·Yuichi ShimaKentaro Suzuki
Jul 25, 2019·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Ken McElreaveyAnu Bashamboo
Nov 13, 2019·Nature Communications·Barbara NicolHumphrey H-C Yao
Aug 28, 2020·Frontiers in Endocrinology·Xiaoheng LiRen-Shan Ge
Sep 16, 2020·International Journal of Molecular Sciences·Martin A Estermann, Craig A Smith
Jan 5, 2021·Frontiers in Cell and Developmental Biology·Martin A EstermannCraig A Smith
Apr 24, 2021·Genes & Development·Emily R FrostJessie M Sutherland
May 6, 2021·Cell Reports·Kotaro SasakiMitinori Saitou
Mar 5, 2021·Endocrinology·Andrew T MajorCraig A Smith
Nov 13, 2020·Physiological Reviews·Yoshitaka NagahamaMasaru Nakamura
May 19, 2021·Seminars in Cell & Developmental Biology·Xiaoheng LiRen-Shan Ge
Jul 16, 2021·Frontiers in Cell and Developmental Biology·Pauline SararolsSerge Nef

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