Deciphering cellular morphology and biocompatibility using polymer microarrays.

Biomedical Materials
Salvatore PernagalloMark Bradley

Abstract

A quantitative and qualitative analysis of cellular adhesion, morphology and viability is essential in understanding and designing biomaterials such as those involved in implant surfaces or as tissue-engineering scaffolds. As a means to simultaneously perform these studies in a high-throughput (HT) manner, we report a normalized protocol which allows the rapid analysis of a large number of potential cell binding substrates using polymer microarrays and high-content fluorescence microscopy. The method was successfully applied to the discovery of optimal polymer substrates from a 214-member polyurethane library with mouse fibroblast cells (L929), as well as simultaneous evaluation of cell viability and cellular morphology. Analysis demonstrated high biocompatibility of the binding polymers and permitted the identification of several different cellular morphologies, showing that specific polymer interactions may provoke changes in cell shape. In addition, SAR studies showed a clear correspondence between cellular adhesion and polymer structure. The approach can be utilized to perform multiple experiments (up to 1024 single experiments per slide) in a highly reproducible manner, leading to the generation of vast amounts of data in ...Continue Reading

References

Dec 15, 1967·Science·J W Boretos, W S Pierce
Jul 16, 1999·Journal of Biomaterials Applications·R J Zdrahala, I J Zdrahala
Jan 14, 2000·Nature Biotechnology·D Luo, W M Saltzman
Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eben AlsbergDavid J Mooney
Sep 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Howard Y ChangPatrick O Brown
Aug 19, 2003·Biomaterials·Heungsoo ShinAntonios G Mikos
Oct 2, 2003·Nature Materials·Henning Sirringhaus
Nov 19, 2003·Nature Materials·Amy C Richards GraysonRobert Langer
Mar 17, 2004·Biomaterials·E A RothT Boland
Apr 2, 2004·Nature·Robert Langer, David A Tirrell
Jun 15, 2004·Nature Biotechnology·Daniel G AndersonRobert Langer
Sep 28, 2004·Science·Daniel G AndersonRobert Langer
May 17, 2006·Chemical Communications : Chem Comm·Guilhem TourniaireMark Bradley
Jul 1, 2006·Biomaterials·Alexandra MantMark Bradley
Jul 20, 2006·Nature Reviews. Molecular Cell Biology·Rainer Pepperkok, Jan Ellenberg
Dec 15, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Michael J SherrattCay M Kielty
May 8, 2007·ACS Chemical Biology·Ratmir DerdaLaura L Kiessling

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Citations

Jul 23, 2013·Biomaterials·Soraya Rasi GhaemiNicolas H Voelcker
Feb 9, 2012·Journal of Visualized Experiments : JoVE·Andrew L HookMorgan R Alexander
Jan 20, 2012·Water Research·Mei WuMark Bradley
Oct 10, 2009·Biomaterials·Andrew L HookMorgan R Alexander
Jul 9, 2009·Journal of Biomedical Materials Research. Part a·Ian S MillerWilliam M Gallagher
Sep 1, 2009·Biotechnology and Bioengineering·Magdalena M MahlstedtChris Denning
Nov 28, 2012·Advanced Healthcare Materials·Salvatore PernagalloMark Bradley
Mar 18, 2009·Tissue Engineering. Part B, Reviews·Anthony PetersJason A Burdick
Jan 22, 2009·Lab on a Chip·Salvatore PernagalloMark Bradley
Feb 7, 2012·Environmental Science & Technology·Harry PickeringHelen Bridle

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