Deciphering colistin heteroresistance in Acinetobacter baumannii clinical isolates from Wenzhou, China.

The Journal of Antibiotics
Lijiang ChenTieli Zhou

Abstract

This study aimed to investigate the characteristics and mechanisms responsible for heteroresistance to colistin in Acinetobactor baumannii clinical isolates from a Chinese teaching hospital. Five hundred and seventy-six nonduplicate A. baumannii clinical isolates isolated from 2014 to 2015 were tested. Colistin heteroresistance was determined using population analysis profiles (PAPs). Susceptibility testing was conducted using the broth microdilution method (BMD). The ability to form biofilm formation was determined using 96-well flat bottom microtiter plates. Time-kill assays were also conducted. PCR and sequencing were used to detect the presence of resistant genes. Expression levels of efflux pump genes were determined by qRT-PCR. LPS analysis was conducted by SDS-PAGE. Lipid A characteristic were determined via MALDI-TOF MS. Nine colistin heteroresistant A. baumannii clinical isolates which were selected by PAPs, exhibited multidrug-resistant phenotypes. The microplate biofilm assay revealed that colistin heteroresistant A. baumannii clinical isolates had weaker biofilm formation capacity than A. baumannii ATCC19606. Colistin-heteroresistant A. baumannii isolates exhibited regrowth after 12 h at 0.5 × MIC, 1 × MIC, and 2 × ...Continue Reading

References

Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Mar 4, 2000·Journal of Microbiological Methods·S StepanovicM Svabic-Vlahovic
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Jian LiLisa Liolios
Oct 24, 2007·Antimicrobial Agents and Chemotherapy·Joshua S HawleyJames H Jorgensen
Jun 17, 2009·Antimicrobial Agents and Chemotherapy·Mark D AdamsRobert A Bonomo
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Jennifer H MoffattJohn D Boyce
Mar 16, 2011·Antimicrobial Agents and Chemotherapy·Jennifer H MoffattJohn D Boyce
Dec 23, 2011·Journal of Clinical Microbiology·Kuei-Hsiang HungJiunn-Jong Wu
Mar 24, 2012·The Journal of Antimicrobial Chemotherapy·Yun CaiNan Bai
Jan 10, 2013·Methods in Molecular Biology·Jessica V HankinsM Stephen Trent
Jul 4, 2013·Annals of Clinical Microbiology and Antimicrobials·Juliana BarinAlexandre Prehn Zavascki
Jan 6, 2015·The Journal of Microbiology·Young Kyoung Park, Kwan Soo Ko
Jan 30, 2015·Jundishapur Journal of Microbiology·Mojtaba MoosavianAmir Peymani
Oct 22, 2015·Expert Review of Anti-infective Therapy·Yiying CaiAndrea L Kwa
Mar 10, 2016·Antimicrobial Agents and Chemotherapy·Wentao NiYouning Liu
Oct 12, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Angeliki MavroidiEvangelia D Platsouka
Nov 23, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I CaniauxM F Gros
Apr 26, 2017·International Journal of Antimicrobial Agents·Elias DahdouhMonica Suárez
Sep 13, 2017·Current Infectious Disease Reports·Pavithra Srinivas, Kaitlyn Rivard
Jun 27, 2019·Nature Reviews. Microbiology·Dan I AnderssonKarin Hjort
Aug 7, 2019·Antimicrobial Agents and Chemotherapy·Jie LinTieli Zhou

❮ Previous
Next ❯

Citations

Feb 3, 2021·Critical Reviews in Microbiology·Susana Patrícia LopesMaria Olívia Pereira

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse transcription PCR
PCR

Software Mentioned

flexAnalysis
SPSS
BLAST
flexControl

Related Concepts

Related Feeds

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.