Deciphering pollutants removal mechanisms and genetic responses to ampicillin stress in simultaneous heterotrophic nitrification and aerobic denitrification (SHNAD) process treating seawater-based wastewater

Bioresource Technology
Haoming WangGuanghao Chen

Abstract

Pollutants removal and genetic responses of simultaneous heterotrophic nitrification and aerobic denitrification (SHNAD) treating seawater-based wastewater were studied under ampicillin stress. Marine SHAND bacteria exhibited good tolerance to 10 mg/L ampicillin with nitrogen removal efficiency and organics removal efficiency of 94.5% and 82.6%, respectively. Besides, the half-inhibitory concentration of ampicillin on marine SHAND bacteria was 50 mg/L. The relative abundances of antibiotic resistance genes (ARGs) first decreased and then increased with ampicillin addition. The blaVIM played an important role to resist 25 mg/L ampicillin, which contributed to the recovery of pollutants removal. BlaSHV and blaTEM dominated ARG subtypes, which accounted for 96.6% of ARGs abundance. At 50 mg/L ampicillin, reactive oxygen species (ROS) production and cell numbers of apoptosis increased by 47.9% and 367.5%, respectively. The overproduction of ROS was stimulated by ampicillin, which caused bacterial cell apoptosis. Marine SHNAD bacteria produced more extracellular polymeric substances to resist ampicillin.

References

Sep 7, 2007·Cell·Michael A KohanskiJames J Collins
Apr 9, 2013·Journal of Environmental Management·Ewa Korzeniewska, Monika Harnisz
Feb 21, 2018·Microbiological Research·Stijn H Peeters, Marien I de Jonge
Apr 24, 2018·Trends in Pharmacological Sciences·Lin-Cheng JuMichael W Crowder
Sep 3, 2018·Microbiological Research·Li LingzhiZhou Xiaohui
Jun 4, 2019·Water Research·Tim HülsenDamien J Batstone
Jun 28, 2019·Environmental Science & Technology·Akashdeep Singh OberoiHui Lu
Nov 27, 2019·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Eloise Müller-SchulteSören L Becker

❮ Previous
Next ❯

Citations

Jul 16, 2021·Chemosphere·Tongchai SriwiriyaratPrayoon Fongsatitkul

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis