Deciphering the anti-Parkinson's activity of sulfated polysaccharides from Chlamydomonas reindertaii on the a-synuclein mutants A30P, A53T, E46K, E57K and E35K

Journal of Biochemistry
Gitanjali P PanigrahiSinjan Choudhary

Abstract

Parkinsonism-linked mutations in alanine and glutamic acid residues of the pre-synaptic protein α-Synuclein affect specific tertiary interactions essential for stability of the native state and make it prone to more aggregation. Many of the currently available drugs used for treatment of Parkinson's disease (PD) are not very effective and are associated with multiple side effects. Recently, marine algae has been reported to have sulfated polysaccharides which offers multiple pharmaceutical properties. With this background, we have isolated sulfated polysaccharides from Chlamydomonas reinhardtii (Cr-SPs) and investigated their effects on inhibition of fibrillation/aggregation of α-Syn mutants through a combination of spectroscopic and microscopic techniques. The kinetics of α-Syn fibrillation establishes that Cr-SPs are very effective in inhibiting fibrillation of α-Syn mutants. The morphological changes associated with the fibrillation/aggregation process have been monitored by transmission electron microscopy. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis gel-image suggests that Cr-SPs increase the amount of soluble protein after completion of the fibrillation/aggregation process. The circular dichroism results sh...Continue Reading

References

Mar 1, 1992·The Biochemical Journal·M Cardamone, N K Puri
Jan 2, 1992·Nature·M J Gething, J Sambrook
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·E H KooJ W Kelly
Jan 8, 2000·Analytical Biochemistry·J T Pelton, L R McLean
Jul 1, 1962·The Biochemical Journal·K S DODGSON, R G PRICE
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Nov 1, 2003·Science·A B SingletonK Gwinn-Hardy
Apr 23, 2004·Biological Procedures Online·Melanie OhiThomas Walz
Jan 6, 2005·The Journal of Biological Chemistry·Eric A GreenbaumBenoit I Giasson
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T M A SilvaE L Leite
Jun 1, 2007·Journal of Agricultural and Food Chemistry·Takayuki TsukuiKazuo Miyashita
Aug 21, 2007·Biotechnology and Bioengineering·Christopher J Roberts
Aug 13, 2008·International Journal of Biological Macromolecules·Kausik ChattopadhyayBimalendu Ray
Dec 5, 2008·Journal of Agricultural and Food Chemistry·Yong LiSe-Kwon Kim
Jul 8, 2009·Biochemical and Biophysical Research Communications·Sonu KumarDurai Sundar
Sep 22, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·L S CostaH A O Rocha
Dec 23, 2009·International Journal of Biological Macromolecules·Tingting HuShusheng Wang
Apr 20, 2010·Biochimica Et Biophysica Acta·Matthew Biancalana, Shohei Koide
Dec 15, 2010·International Journal of Biological Macromolecules·Supatra Karnjanapratum, SangGuan You
Feb 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Beate WinnerRoland Riek
Feb 23, 2011·Marine Drugs·Rafael Barros Gomes CamaraHugo Alexandre Oliveira Rocha
May 14, 2011·Marine Drugs·Guangling JiaoH Stephen Ewart
Jun 16, 2011·Marine Drugs·Ratih Pangestuti, Se-Kwon Kim
Jul 13, 2011·Marine Drugs·Leandro Silva CostaHugo Alexandre Oliveira Rocha
Sep 1, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Edfranck de Sousa Oliveira VanderleiNorma Maria Barros Benevides
Oct 12, 2011·Basic & Clinical Pharmacology & Toxicology·Chistiane O CouraNorma M B Benevides
Feb 16, 2012·The Journal of Biological Chemistry·Luisel R LemkauChad M Rienstra
Feb 23, 2012·Cold Spring Harbor Perspectives in Medicine·Leonidas Stefanis
Dec 12, 2012·Carbohydrate Polymers·Francisco C N BarrosRegina C M de Paula
Jan 25, 2013·Marine Drugs·Noel Vinay Thomas, Se-Kwon Kim
Aug 21, 2013·Pharmacological Reports : PR·Ana L G QuinderéNorma M B Benevides
Sep 3, 2013·International Journal of Biological Macromolecules·Ping ShaoPeilong Sun
Sep 5, 2013·Vestnik Rossiĭskoĭ akademii meditsinskikh nauk·N N BesednovaT S Zaporozhets
Nov 23, 2013·Journal of Thrombosis and Haemostasis : JTH·A-L G QuinderéP A S Mourão
Mar 29, 2014·Basic & Clinical Pharmacology & Toxicology·José Gerardo CarneiroNorma Maria Barros Benevides
Nov 18, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel Pereira FidelisLeandro Silva Costa
Mar 26, 2015·PloS One·Chistiane Oliveira CouraNorma Maria Barros Benevides
May 20, 2015·Marine Drugs·Maria Filomena de Jesus RaposoRui Manuel Santos Costa de Morais

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Citations

May 21, 2020·Molecular Neurobiology·Aida ZuluetaMarco Trinchera
May 27, 2020·Applied Microbiology and Biotechnology·Jyoti Vishwakarma, Sirisha V L
Aug 28, 2021·Applied Biochemistry and Biotechnology·Jyoti VishwakarmaSirisha L Vavilala

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