Deciphering the biochemical similarities and differences among mouse embryonic stem cells, somatic and cancer cells using ATR-FTIR spectroscopy

The Analyst
Günnur GülerHuseyin Aktug

Abstract

Cellular macromolecules play important roles in cellular behaviors and biological processes. In the current work, cancer (KLN205), normal (MSFs) and mouse embryonic stem cells (mESCs) are compared using ATR-FTIR spectroscopy. Modifications in the composition, concentration, structure and function-related changes in the cellular components were deciphered using the infrared spectra. Our results revealed that cancer and embryonic stem cells are very similar but highly different from the normal cells based on the spectral variations in the protein, lipid, carbohydrate and nucleic acid components. The longest lipid acyl chains exist in mESCs, while cancer cells harbor the lowest lipid amount, short lipid acyl chains, a high content of branched fatty acids and thin cell membranes. The highest cellular growth rate and accelerated cell divisions were observed in the cancer cells. However, the normal cells harbor low nucleic acid and glycogen amounts but have a higher lipid composition. Any defect in the signaling pathways and/or biosynthesis of these cellular parameters during the embryonic-to-somatic cell transition may lead to physiological and molecular events that promote cancer initiation, progression and drug resistance. We conc...Continue Reading

References

Feb 28, 2001·Progress in Biophysics and Molecular Biology·A Barth
Mar 10, 2001·Canadian Journal of Physiology and Pharmacology·A M MelinG Deleris
May 18, 2001·Nature·G I Evan, K H Vousden
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
May 12, 2004·Current Opinion in Plant Biology·Xuemin Wang
Nov 9, 2005·Blood Reviews·Paul H Lerou, George Q Daley
Oct 6, 2007·Biochimica Et Biophysica Acta·Diletta AmiCarlo Alberto Redi
Oct 31, 2008·Talanta·G I DovbeshkoO P Pashchuk
Jun 2, 2010·International Journal of Molecular Medicine·Kaiser AliEric Pellerin
Jun 8, 2012·The Analyst·Allison DerenneErik Goormaghtigh
Sep 22, 2012·Journal of Biophotonics·Donna R WhelanBayden R Wood
Oct 23, 2012·Biochimica Et Biophysica Acta·Allison DerenneErik Goormaghtigh
Oct 29, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Emilia StaniszewskaMalgorzata Baranska
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Sep 4, 2015·Biochimica Et Biophysica Acta·Alix MignoletErik Goormaghtigh
Nov 26, 2015·The Journal of Biological Chemistry·Günnur GülerWerner Mäntele
Feb 18, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christos E Zois, Adrian L Harris
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Huseyin AktugVildan Bozok Cetintas
Jan 4, 2017·Oncotarget·Míriam Tarrado-CastellarnauMarta Cascante
Jan 15, 2017·Molecular and Cellular Endocrinology·Paula Saavedra-GarcíaEric W-F Lam

❮ Previous
Next ❯

Citations

Jun 7, 2019·Journal of Cellular Biochemistry·Berrin OzdilHuseyin Aktug

❮ Previous
Next ❯

Methods Mentioned

BETA
infrared spectroscopy

Software Mentioned

MATLAB
Kinetics
LabSolutions
Excel
OPUS

Related Concepts

Related Feeds

Cancer Metabolism: Therapeutic Targets

Targeting the mechanisms by which cancer cells acquire energy for metabolic needs is a therapeutic target. Discover the latest research on cancer metabolism and therapeutic targets.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.