Deciphering the importance of three key media components in human embryonic stem cell cultures

Biotechnology Letters
Vanessa M Y DingSteve K W Oh

Abstract

Development of a serum free, feeder-free (SFFF) culture platform for human embryonic stem cells (hESC) will be important for the expansion of hESC for future cell therapy applications. However, currently, culture of hESC consists of a combination of basal media, basic fibroblast growth factor (bFGF), serum replacer (SR) and conditioned media (CM) from feeders, and it is unclear which components of the mixture are absolutely critical in the maintenance of hESC. To evaluate the relative contributions of these media components in the development of SFFF culture, each was systematically eliminated and pluripotency assayed by dual embryonic stem cell markers, Oct-4 and TRA-1-60. We concluded that SR was the most critical component in the platform, followed by bFGF and CM produced by feeders, where down-regulation of Oct-4 occurred after 2, 5 and 5 passages, respectively, upon their withdrawal from the complete media.

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Citations

May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jiaxi ZhouFei Wang
Aug 27, 2013·PloS One·Vanessa DingSheng-Hao Chao
Mar 5, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Blaine W Phillips, Jeremy M Crook
Nov 26, 2015·Biochemical and Biophysical Research Communications·Katerina Drakou, Pantelis Georgiades
May 28, 2010·Journal of Cellular Physiology·Vanessa M Y DingAndre B H Choo
Apr 28, 2009·Stem Cell Research·Steve K W OhShaul Reuveny
Mar 6, 2021·The Journal of Biological Chemistry·Adrian Kee Keong TeoRohit N Kulkarni

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