Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa.

Eukaryotic Cell
Jianping SunN Louise Glass

Abstract

Hemicellulose, the second most abundant plant biomass fraction after cellulose, is widely viewed as a potential substrate for the production of liquid fuels and other value-added materials. Degradation of hemicellulose by filamentous fungi requires production of many different enzymes, which are induced by biopolymers or its derivatives and regulated mainly at the transcriptional level through transcription factors (TFs). Neurospora crassa, a model filamentous fungus, expresses and secretes enzymes required for plant cell wall deconstruction. To better understand genes specifically associated with degradation of hemicellulose, we applied secretome and transcriptome analysis to N. crassa grown on beechwood xylan. We identified 34 secreted proteins and 353 genes with elevated transcription on xylan. The xylanolytic phenotype of strains with deletions in genes identified from the secretome and transcriptome analysis of the wild type was assessed, revealing functions for known and unknown proteins associated with hemicellulose degradation. By evaluating phenotypes of strains containing deletions of predicted TF genes in N. crassa, we identified a TF (XLR-1; xylan degradation regulator 1) essential for hemicellulose degradation that...Continue Reading

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Citations

Jan 19, 2013·Analytical and Bioanalytical Chemistry·Roland LudwigLo Gorton
Apr 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Samuel T CoradettiN Louise Glass
Jan 24, 2013·Biotechnology for Biofuels·Elizabeth A Znameroski, N Louise Glass
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Mar 25, 2014·Biotechnology for Biofuels·Lilian Dos Santos CastroRoberto Nascimento Silva
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Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Lori B HubermanN Louise Glass

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