Decolonisation of MRSA, S. aureus and E. coli by cold-atmospheric plasma using a porcine skin model in vitro.

PloS One
Tim MaischJulia L Zimmermann

Abstract

In the last twenty years new antibacterial agents approved by the U.S. FDA decreased whereas in parallel the resistance situation of multi-resistant bacteria increased. Thus, community and nosocomial acquired infections of resistant bacteria led to a decrease in the efficacy of standard therapy, prolonging treatment time and increasing healthcare costs. Therefore, the aim of this work was to demonstrate the applicability of cold atmospheric plasma for decolonisation of Gram-positive (Methicillin-resistant Staphylococcus aureus (MRSA), Methicillin-sensitive Staphylococcus aureus) and Gram-negative bacteria (E. coli) using an ex vivo pig skin model. Freshly excised skin samples were taken from six month old female pigs (breed: Pietrain). After application of pure bacteria on the surface of the explants these were treated with cold atmospheric plasma for up to 15 min. Two different plasma devices were evaluated. A decolonisation efficacy of 3 log(10) steps was achieved already after 6 min of plasma treatment. Longer plasma treatment times achieved a killing rate of 5 log(10) steps independently from the applied bacteria strains. Histological evaluations of untreated and treated skin areas upon cold atmospheric plasma treatment wit...Continue Reading

References

Sep 1, 1986·Journal of Clinical Microbiology·L W BushJ H White
Apr 2, 1998·Journal of Industrial Microbiology & Biotechnology·K Kelly-WintenbergP P Tsai
Nov 7, 2002·Archives of Dermatological Research·Vivien SchachtChristoph Abels
Jan 8, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·John M BoyceUNKNOWN Healthcare Infection Control Practices Advisory Committee. Society for Healthcare Epidemiology of America. Association for P
Jan 5, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R Monina KlevensUNKNOWN National Nosocomial Infections Surveillance System
May 17, 2006·Genesis : the Journal of Genetics and Development·M KretzK Willecke
Apr 11, 2007·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Wilfried MeyerJohannes Seeger
May 10, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·T MaischC Abels
Jan 4, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Brad SpellbergUNKNOWN Infectious Diseases Society of America
Apr 19, 2008·The Journal of Infectious Diseases·Louis B Rice
May 31, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M BuehlmannA F Widmer
Jan 31, 2009·The New England Journal of Medicine·Cesar A Arias, Barbara E Murray
Jun 30, 2009·American Journal of Infection Control·Monica L BurtsJonathan A McCullers
Nov 1, 1938·The Journal of Hygiene·A A MilesJ O Irwin
Oct 5, 2010·Nitric Oxide : Biology and Chemistry·Joerg LiebmannVictoria Kolb-Bachofen

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Citations

Mar 19, 2014·International Journal of Antimicrobial Agents·Anne Mai-ProchnowKostya Ken Ostrikov
May 15, 2013·Expert Review of Medical Devices·Georg IsbaryJulia L Zimmermann
Jan 23, 2016·Journal of Biophotonics·Anita GollmerCristina Flors
Dec 10, 2014·Biomolecules & Therapeutics·Beate HaertelUlrike Lindequist
Mar 27, 2013·Experimental Dermatology·Stephanie ArndtAnja-Katrin Bosserhoff
Apr 10, 2016·Archives of Biochemistry and Biophysics·Tomohiro OkadaShinya Kumagai
Sep 24, 2015·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S Karrer, S Arndt
Apr 15, 2015·Biointerphases·Svetlana A ErmolaevaAlexander L Gintsburg
Jul 15, 2016·Photomedicine and Laser Surgery·Mauricio DujowichJames F X Wellehan
Apr 4, 2017·Environmental and Molecular Mutagenesis·T MaischS Karrer
Nov 20, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anita GollmerAndreas Späth
Jun 20, 2018·Applied and Environmental Microbiology·Li GuoMichael G Kong
May 15, 2012·Applied and Environmental Microbiology·Tobias G KlämpflHans-Ulrich Schmidt
Jan 21, 2014·Applied and Environmental Microbiology·Orla J CahillHilary Humphreys
Nov 9, 2019·Journal of Bone and Joint Infection·Guido W Van OijenEsther M M Van Lieshout
Aug 13, 2020·International Journal of Dermatology·Peter C Friedman
May 5, 2016·Advances in Therapy·Jesús Gay-MimbreraJuan Ruano
Jul 4, 2018·Scientific Reports·Christin SchneiderAnja-Katrin Bosserhoff
Aug 30, 2019·Materials·Fatemeh RezaeiNathalie De Geyter
Nov 14, 2018·Scientific Reports·Kedar PaiSundararajan V Madihally
Mar 10, 2017·Frontiers in Cellular and Infection Microbiology·Saga HelgadóttirIvan Mijakovic
Feb 16, 2021·Applied Microbiology and Biotechnology·Bouke BoekemaEsther Middelkoop

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Methods Mentioned

BETA
biopsies

Software Mentioned

miniFlatPlaSter
FlatPlaSter
GraphPad
Prism
Pro Plus
Image

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