Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression.

PLoS Computational Biology
Christopher C PoirierPablo A Iglesias

Abstract

Cytokinesis occurs through the coordinated action of several biochemically-mediated stresses acting on the cytoskeleton. Here, we develop a computational model of cellular mechanics, and using a large number of experimentally measured biophysical parameters, we simulate cell division under a number of different scenarios. We demonstrate that traction-mediated protrusive forces or contractile forces due to myosin II are sufficient to initiate furrow ingression. Furthermore, we show that passive forces due to the cell's cortical tension and surface curvature allow the furrow to complete ingression. We compare quantitatively the furrow thinning trajectories obtained from simulation with those observed experimentally in both wild-type and myosin II null Dictyostelium cells. Our simulations highlight the relative contributions of different biomechanical subsystems to cell shape progression during cell division.

References

May 10, 2000·Current Biology : CB·I WeberG Gerisch
May 2, 2001·Nature Reviews. Molecular Cell Biology·J A Spudich
Feb 28, 2002·BMC Cell Biology·Douglas N RobinsonJames A Spudich
Mar 19, 2005·Science·Michael Glotzer
Apr 9, 2005·Trends in Cell Biology·Elizabeth M ReichlDouglas N Robinson
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wendy Zhang, Douglas N Robinson
May 13, 2005·Nature·Cornelis StormPaul A Janmey
Jun 10, 2005·Molecular Biology of the Cell·Masamitsu KanadaTaro Q P Uyeda
Dec 2, 2005·Current Opinion in Cell Biology·Alex Mogilner
Jun 8, 2006·Annual Review of Biochemistry·Ulrike S EggertChristine M Field
Oct 10, 2006·Current Biology : CB·Janet C EfflerDouglas N Robinson
Oct 20, 2006·Molecular Biology of the Cell·Edelyn OcttavianiDouglas N Robinson
Aug 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan C Del AlamoJuan C Lasheras
Nov 6, 2007·Biophysical Journal·Roie Shlomovitz, Nir S Gov
Feb 12, 2008·Current Biology : CB·Alice Y Pollitt, Robert H Insall
Mar 29, 2008·Current Biology : CB·Elizabeth M ReichlDouglas N Robinson
Apr 5, 2008·Journal of Cell Science·Yoshiaki Iwadate, Shigehiko Yumura
Jul 5, 2008·Biophysical Journal·Ilka B BischofsUlrich S Schwarz
Jul 26, 2008·BMC Systems Biology·Liu YangPablo A Iglesias
Sep 8, 2009·Trends in Cell Biology·Patrick Steigemann, Daniel W Gerlich
Dec 4, 2009·Molecular Biology of the Cell·Ruedi MeiliJuan C Lasheras
Jul 27, 2010·Biophysical Journal·Mathias BuenemannLeonard M Sander
Aug 17, 2010·Seminars in Cell & Developmental Biology·Alexandra SurcelDouglas N Robinson
Sep 3, 2010·Nature·Clemens CabernardChris Q Doe
May 17, 2011·PLoS Computational Biology·Doron KabasoNir S Gov
Mar 2, 2012·Molecular Biology of the Cell·Yee-Seir KeeDouglas N Robinson

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Citations

Aug 16, 2014·Quarterly Reviews of Biophysics·David Sept, Anders E Carlsson
Nov 1, 2012·Molecular Biology of the Cell·Douglas N Robinson, Pablo A Iglesias
Oct 4, 2013·Molecular Biology of the Cell·Tara C SmithElizabeth J Luna
Oct 22, 2013·Nature Cell Biology·Chuan-Hsiang HuangPeter N Devreotes
Feb 24, 2015·Current Biology : CB·Vasudha Srivastava, Douglas N Robinson
Apr 11, 2012·Experimental Cell Research·Krithika MohanDouglas N Robinson
Dec 4, 2014·Biophysical Journal·Tamara C BidoneDimitrios Vavylonis
Oct 3, 2012·Cytoskeleton·Mohan K BalasubramanianKian-Hong Ng
Jul 5, 2012·Cytoskeleton·Hoku West-Foyle, Douglas N Robinson
Aug 13, 2013·Biophysical Journal·Inês Mendes PintoRong Li
Aug 1, 2015·Journal of the Royal Society, Interface·Krithika MohanPablo A Iglesias
Oct 21, 2015·Seminars in Cell & Developmental Biology·Vasudha SrivastavaDouglas N Robinson
Nov 5, 2016·Molecular Biology of the Cell·Alex Mogilner, Angelika Manhart
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alka ChoudharyMark E Burkard
Jan 26, 2017·Biophysical Journal·Eric S Schiffhauer, Douglas N Robinson
Feb 19, 2016·International Journal for Numerical Methods in Biomedical Engineering·Jia Zhao, Qi Wang
Dec 19, 2018·Journal of Cell Science·Priyanka KothariDouglas N Robinson
Jan 19, 2019·The Journal of Cell Biology·Eric S SchiffhauerDouglas N Robinson
May 22, 2019·Biomolecules·Joana LeiteAna Xavier Carvalho
Feb 2, 2013·Physical Review Letters·Hirokazu Tanimoto, Masaki Sano
Jul 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Alexandra Surcel, Douglas N Robinson
Sep 4, 2019·Journal of Cell Science·Priyanka KothariDouglas N Robinson
May 28, 2020·Molecular Biology of the Cell·Kelly M HennesseyAlexander R Paredez
Dec 23, 2016·Cold Spring Harbor Perspectives in Biology·Michael Glotzer
Dec 14, 2018·F1000Research·Yinan Liu, Douglas Robinson
Jan 17, 2019·Annual Review of Biochemistry·Thomas D Pollard, Ben O'Shaughnessy
Jul 31, 2019·Biology·Vikash VermaThomas J Maresca
Jul 7, 2020·Frontiers in Cell and Developmental Biology·Ly T S Nguyen, Douglas N Robinson
Sep 19, 2019·Physical Biology·David CaballeroJosep Samitier
Jul 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·William R HardinAlexander R Paredez
Nov 17, 2016·Biophysical Journal·Valerie C CoffmanAdriana T Dawes
Dec 12, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Kathleen T DiNapoliPablo A Iglesias
Oct 24, 2021·Biophysical Journal·Kathleen T DiNapoliPablo A Iglesias

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Methods Mentioned

BETA
GTPases

Software Mentioned

Level Set Toolbox
Matlab
ImageJ

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