Decorated rods: a "bottom-up" self-assembly of monomolecular DNA complexes

The Journal of Physical Chemistry. B
Jason DeRoucheyJoachim O Rädler

Abstract

Fluorescence correlation spectroscopy (FCS) and gel electrophoresis measurements are performed to investigate both the number and size of complexes of linear double-stranded DNA (dsDNA) fragments with 1:1 diblock copolymers consisting of a cationic moiety, branched polyethyleneimine (bPEI) of 2, 10, or 25 kDa, covalently bound to a neutral shielding moiety, poly(ethylene glycol) (PEG; 20 kDa). By systematically decreasing the bPEI length, the PEG grafting density along the DNA chain can be directly controlled. For 25 and 10 kDa bPEI-PEG copolymers, severe aggregation is observed despite the presence of the shielding PEG. Upon decreasing the bPEI length to 2 kDa, controlled self-assembly of monomolecular DNA nanoparticles is observed. The resulting complexes are in quantitative agreement with a theoretical model based on a single DNA encased in a dense PEG polymer brush layer. The resulting PEGylated complexes show high stability against both salt and protein and hence are of potential use for in vivo gene delivery studies.

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Citations

Aug 3, 2010·Biomacromolecules·Daniel HönigJoachim O Rädler
Dec 19, 2013·Journal of the American Chemical Society·Antons SizovsJin Wang
Jun 1, 2012·ACS Nano·Christian DohmenErnst Wagner
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Jan 29, 2008·Biomacromolecules·Jason DeRoucheyJoachim O Rädler

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