Decorating bacteria with self-assembled synthetic receptors

Nature Communications
Naama Lahav-MankovskiDavid Margulies

Abstract

The responses of cells to their surroundings are mediated by the binding of cell surface proteins (CSPs) to extracellular signals. Such processes are regulated via dynamic changes in the structure, composition, and expression levels of CSPs. In this study, we demonstrate the possibility of decorating bacteria with artificial, self-assembled receptors that imitate the dynamic features of CSPs. We show that the local concentration of these receptors on the bacterial membrane and their structure can be reversibly controlled using suitable chemical signals, in a way that resembles changes that occur with CSP expression levels or posttranslational modifications (PTMs), respectively. We also show that these modifications can endow the bacteria with programmable properties, akin to the way CSP responses can induce cellular functions. By programming the bacteria to glow, adhere to surfaces, or interact with proteins or mammalian cells, we demonstrate the potential to tailor such biomimetic systems for specific applications.

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Citations

Oct 2, 2020·Advanced Science·Pere MongeAlexander N Zelikin
Jan 12, 2021·Chemical Communications : Chem Comm·Joydev HataiDavid Margulies
Nov 24, 2020·Beilstein Journal of Organic Chemistry·Pragati Kishore PrasadDavid Margulies
Jun 25, 2021·Chemical Science·Qian TianMingxu You
Aug 14, 2020·Journal of the American Chemical Society·Ronny Peri-NaorDavid Margulies

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Methods Mentioned

BETA
flow
flow cytometry
fluorescence microscopy
biosensors
FACS

Software Mentioned

Tree Star
BD
FACS Diva
FlowJo

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