Decorin and chondroitin-6 sulfate inhibit B16V melanoma cell migration and invasion by cellular acidification

Journal of Cellular Physiology
Christian StockDaniela G Seidler


In vivo, cells are embedded in an environment generated and maintained by multiple cell-cell and cell-matrix interactions. While transiting the dermis metastasizing melanoma cells interact with the extracellular matrix (ECM) and fibroblasts. To study the roles of ECM components and fibroblasts in melanoma (B16V) cell migration and invasion, we established a co-culture system consisting of fibroblasts, their collagen-rich matrix and B16V cells. The crosstalk between B16V cells and fibroblasts was indicated by a clear increase in release and activity of matrix-metallo-protease-2. Time-lapse microscopy revealed that in B16V cells exposed to either decorin or chondroitin sulfates migration and invasion decreased by more than 50%. Decorin led to a reversible, chondroitin-6-sulfate to an irreversible, cytosolic acidification of B16V cells. Interestingly, decorin lowered NHE1 activity whereas chondroitin-6-sulfate did not. Furthermore, decorin and chondroitin-6-sulfate also acidified the pH at the cell surface which might prevent migration due to strong adhesion. In conclusion, the present co-culture system is an appropriate tool to analyze migration, invasion, and MMP release depending on cell-matrix interactions and the crosstalk be...Continue Reading


Jan 1, 1992·Rheumatology International·A BaiciF J Wagenhäuser
Apr 22, 1991·International Journal of Cancer. Journal International Du Cancer·G N van MuijenD J Ruiter
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J PouysségurS Paris
Aug 1, 1994·Molecular and Cellular Biology·A G BatzerJ Schlessinger
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M SantraR V Iozzo
Apr 1, 1994·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·H KähnertK Kleesiek
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·R V Iozzo
Jun 9, 1998·Journal of Cellular Physiology·V RantaA Orpana
May 26, 1999·The Journal of Membrane Biology·S KirschbergerW von Engelhardt
Jul 6, 2000·The Journal of Membrane Biology·O N Kovbasnjuk, K R Spring
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L SchaeferH Kresse
Dec 19, 2001·Journal of Cellular Physiology·H Kresse, E Schönherr
Apr 3, 2002·The Journal of Clinical Investigation·Alla Danilkovitch-Miagkova, Berton Zbar
May 7, 2002·International Journal of Cancer. Journal International Du Cancer·Takuro KanekuraTamotsu Kanzaki
May 29, 2002·Oncogene·Charles C ReedRenato V Iozzo
Jul 11, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ayman Al Haj ZenBruno Gogly
Mar 24, 2004·Critical Reviews in Oncology/hematology·Yanusz Wegrowski, Francois-Xavier Maquart
Apr 17, 2004·The Journal of Investigative Dermatology·Toon F C M SmetsersToin H van Kuppevelt
Oct 27, 2004·Journal of Dental Research·X H ZouG W Yip
Nov 6, 2004·Journal of Vascular Research·Elke SchönherrHans Kresse
Feb 4, 2005·Oncogene·Charles C ReedRenato V Iozzo
Mar 23, 2005·Biochimie·Uta B HofmannJürgen C Becker
Jun 11, 2005·The Journal of Physiology·Christian StockAlbrecht Schwab
Jun 14, 2005·Biochemical and Biophysical Research Communications·Daniela G SeidlerElke Schönherr
Jan 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kristen R Taylor, Richard L Gallo
Jun 1, 2006·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Stefanie LöffekCornelia Mauch
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joseph P R O OrgelTim J Wess
Jan 24, 2007·Advances in Pharmacology·Yanusz Wegrowski, François-Xavier Maquart
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Christian StockAlbrecht Schwab
Mar 11, 2008·European Journal of Cell Biology·Christian StockStephan J Reshkin
May 9, 2008·The Journal of Biological Chemistry·Liliana Schaefer, Renato V Iozzo
May 13, 2008·International Journal of Cancer. Journal International Du Cancer·Wei GongZuo-Hua Feng

❮ Previous
Next ❯


Feb 9, 2012·Onkologie·Marek PytliakViola Mechírová
Oct 12, 2014·FEBS Letters·Katarzyna PietraszekYanusz Wegrowski
Nov 3, 2015·Advanced Drug Delivery Reviews·Thomas NeillRenato V Iozzo
Oct 13, 2012·European Journal of Cell Biology·David Denis Sofeu FeugaingManuela Viola
Apr 6, 2013·Journal of Cellular Physiology·Nadine Kathagen, Peter Prehm
Jun 17, 2016·Molecular & Cellular Oncology·Thomas NeillRenato V Iozzo
Jan 23, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Katerina NikolovskaDaniela G Seidler
Feb 11, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Hannu JärveläinenThomas N Wight
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Liliana Schaefer
Aug 11, 2012·Current Opinion in Structural Biology·Daniela G Seidler
Mar 21, 2017·Seminars in Cancer Biology·Leticia Oliveira-FerrerKarin Milde-Langosch
Jan 21, 2017·PloS One·Katerina NikolovskaDaniela G Seidler
Jul 25, 2019·Frontiers in Cell and Developmental Biology·Sameera NallanthighalDong-Joo Cheon
Jun 20, 2020·International Journal of Biological Macromolecules·Lais C G F PalharesSuely F Chavante
Jun 29, 2021·Frontiers in Oncology·Elisa Helena Farias JandreyÉrico Tosoni Costa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.