Decorin, versican, and biglycan gene expression by keloid and normal dermal fibroblasts: differential regulation by basic fibroblast growth factor

Experimental Cell Research
E M TanG R Dodge

Abstract

Basic fibroblast growth factor (bFGF) is a multipotential heparin-binding factor that belongs to the fibroblast growth factor (FGF) family. The FGFs demonstrate a wide spectrum of biologic activities in vivo and in vitro. In this study, we investigated the potential of bFGF to regulate the expression of various dermal extracellular matrix proteoglycans and type I collagen mRNAs in cultured human fibroblasts from keloid, which is a prototype of dermal fibrosis, and normal skin tissue. We report that bFGF upregulates the expression of the decorin gene in normal and keloid fibroblasts. In contrast, the expression of biglycan is downregulated by bFGF. The mRNA steady-state level of versican, a large proteoglycan, is not altered by bFGF. Type I collagen gene expression is downregulated substantially in keloid and normal fibroblasts by bFGF. The results suggest that the expression of the proteoglycan genes are uncoordinately regulated and that the gene expression of type I collagen and biglycan is coordinately downregulated. The results also demonstrate that keloid fibroblasts respond similarly as do normal fibroblasts to bFGF in the regulation of proteoglycan and collagen expression.

Citations

Sep 1, 1996·The British Journal of Dermatology·N HunzelmannT Krieg
Feb 1, 2005·Experimental Dermatology·Toshiyuki Yamamoto, Kiyoshi Nishioka
Jan 20, 2012·The Biochemical Journal·David A CarrinoArnold I Caplan
Apr 4, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rebecca M HarmanGerlinde R Van de Walle
Oct 28, 2003·The Journal of Dermatology·Yoshihiro NomuraMasafumi Tsujimoto
Dec 24, 2016·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Shih-Yi YangShiow-Shuh Chuang
Dec 13, 2016·Archives of Dermatological Research·Dutsadee SutthoGwenaël Rolin
Mar 14, 2000·Cell Death and Differentiation·C C ChipevM Simon
Apr 1, 1997·Pediatric Research·K A Veness-MeehanA D Stiles
Dec 1, 2001·Clinical and Experimental Hypertension : CHE·Y AyadaT Tsuji
Jun 21, 2019·Cells·Alexandra L RippaEkaterina A Vorotelyak
Feb 18, 2020·Frontiers in Pharmacology·Xiaoxiao PangZhong Zheng

Related Concepts

VCAN protein, human
DCN protein, human
BGN protein, human
Zyderm
Fibroblasts
Keloid
Chondroitin Sulfate Proteoglycans
Proteoglycan Type H
Poly(A) Tail
Gene Expression

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