Decoupling the effects of hydrophilic and hydrophobic moieties at the neuron-nanofibre interface.

Chemical Science
Adam D MartinPall Thordarson

Abstract

Peptide-based nanofibres are a versatile class of tunable materials with applications in optoelectronics, sensing and tissue engineering. However, the understanding of the nanofibre surface at the molecular level is limited. Here, a series of homologous dilysine-diphenylalnine tetrapeptides were synthesised and shown to self-assemble into water-soluble nanofibres. Despite the peptide nanofibres displaying similar morphologies, as evaluated through atomic force microscopy and neutron scattering, significant differences were observed in their ability to support sensitive primary neurons. Contact angle and labelling experiments revealed that differential presentation of lysine moieties at the fibre surface did not affect neuronal viability; however the mobility of phenylalanine residues at the nanofibre surface, elucidated through solid- and gel-state NMR studies and confirmed through tethered bilayer lipid membrane experiments, was found to be the determining factor in governing the suitability of a given peptide as a scaffold for primary neurons. This work offers new insights into characterising and controlling the nanofibre surface at the molecular level.

References

Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·T C HolmesS Zhang
Feb 20, 2003·Protein Science : a Publication of the Protein Society·Francesca M Marassi, Stanley J Opella
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ana Carolina ZeriStanley J Opella
Dec 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Franc AvbeljRobert L Baldwin
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Apr 11, 2006·Biomacromolecules·Benedict LawChing-Hsuan Tung
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lisa Haines-ButterickJoel P Schneider
Nov 20, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Lin ChenDave J Adams
Sep 16, 2010·Chemistry of Materials : a Publication of the American Chemical Society·Cole A DeforestKristi S Anseth
Jan 29, 2011·Journal of Colloid and Interface Science·Sila ToksozMustafa O Guler
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Katelyn Nagy-SmithRobert Tycko
Jan 1, 2016·ACS Applied Materials & Interfaces·Yuqiao SunWutian Wu
Jul 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Chun YangKristi S Anseth
Nov 4, 2016·Advanced Materials·Danielle Lynne Taylor, Marc In Het Panhuis
Oct 22, 2016·Acta Biomaterialia·Luis M De Leon-RodriguezMargaret A Brimble
Feb 14, 2017·Accounts of Chemical Research·Amanda N Moore, Jeffrey D Hartgerink
Feb 25, 2017·Science·Einav Tayeb-FligelmanMeytal Landau
Jun 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Charles G CranfieldBruce Cornell
Jun 21, 2017·Biomacromolecules·Laura L E MearsDave J Adams
Sep 9, 2017·Nature Communications·Mian BiLars M Ittner
Jan 10, 2018·Nature Nanotechnology·Hajime ShigemitsuItaru Hamachi
Feb 9, 2018·Chemical Society Reviews·Emily R Draper, Dave J Adams
May 12, 2018·Chemical Society Reviews·A LampelT Tuttle
Jul 7, 2018·ACS Applied Materials & Interfaces·Adam D MartinLars M Ittner
Aug 19, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas BerryCharles G Cranfield
Oct 6, 2018·Science·Ronit FreemanSamuel I Stupp
Feb 2, 2019·Science·Takahiro MatsudaJian Ping Gong
May 3, 2019·Journal of the American Chemical Society·Zhihui YaoSamuel H Gellman
May 22, 2019·Nature Materials·Jared A ShadishCole A DeForest

❮ Previous
Next ❯

Citations

Mar 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Biyun SunAdam D Martin
Apr 3, 2020·Nanoscale·Jonathan P WojciechowskiPall Thordarson

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
AFM
Small
angle neutron scattering
small angle neutron scattering
circular dichroism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.