Decoy exosomes provide protection against bacterial toxins

Nature
Matthew D KellerKen Cadwell

Abstract

The production of pore-forming toxins that disrupt the plasma membrane of host cells is a common virulence strategy for bacterial pathogens such as methicillin-resistant Staphylococcus aureus (MRSA)1-3. It is unclear, however, whether host species possess innate immune mechanisms that can neutralize pore-forming toxins during infection. We previously showed that the autophagy protein ATG16L1 is necessary for protection against MRSA strains encoding α-toxin4-a pore-forming toxin that binds the metalloprotease ADAM10 on the surface of a broad range of target cells and tissues2,5,6. Autophagy typically involves the targeting of cytosolic material to the lysosome for degradation. Here we demonstrate that ATG16L1 and other ATG proteins mediate protection against α-toxin through the release of ADAM10 on exosomes-extracellular vesicles of endosomal origin. Bacterial DNA and CpG DNA induce the secretion of ADAM10-bearing exosomes from human cells as well as in mice. Transferred exosomes protect host cells in vitro by serving as scavengers that can bind multiple toxins, and improve the survival of mice infected with MRSA in vivo. These findings indicate that ATG proteins mediate a previously unknown form of defence in response to infect...Continue Reading

References

Oct 19, 2005·The Biochemical Journal·Alexander StoeckPeter Altevogt
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Mar 1, 2008·Science·Katarina TrajkovicMikael Simons
Dec 14, 2012·Nature·Francis AlonzoVictor J Torres
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ashley L DuMontVictor J Torres
Aug 21, 2013·Cell Host & Microbe·Amanda M MarchiandoKen Cadwell
May 14, 2014·Journal of Innate Immunity·Russell E N BeckerJuliane Bubeck Wardenburg
Aug 26, 2014·Nature Cell Biology·Masayuki ShimodaRama Khokha
Feb 17, 2015·Nature Cell Biology·Lyndsay MurrowJayanta Debnath
Mar 31, 2015·Cell Host & Microbe·Katie MaurerKen Cadwell
Dec 8, 2015·Nature Reviews. Microbiology·Matteo Dal Peraro, F Gisou van der Goot
Apr 2, 2016·The Journal of Clinical Investigation·Raghu Kalluri
Dec 10, 2016·The EMBO Journal·Tomonori KimuraVojo Deretic
Apr 20, 2017·Nature Reviews. Microbiology·András N SpaanVictor J Torres
Apr 27, 2017·Seminars in Cell & Developmental Biology·E Sachiko Seilie, Juliane Bubeck Wardenburg
Nov 2, 2017·The Journal of Experimental Medicine·Yu Matsuzawa-IshimotoKen Cadwell
Nov 18, 2017·Annual Review of Immunology·Yu Matsuzawa-IshimotoKen Cadwell

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Citations

Apr 24, 2020·Journal of Cellular Physiology·Bo QinKun Xiong
Jun 12, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mukund VarmaRamnik J Xavier
Sep 18, 2020·American Journal of Reproductive Immunology : AJRI·Yingshi OuyangYoel Sadovsky
Mar 24, 2020·Nature Reviews. Microbiology·Andrea Du Toit
Oct 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lang RaoXiaoyuan Chen
Oct 9, 2020·Chemistry, an Asian Journal·Zejun JiangJianping Li
Jan 5, 2021·Journal of Extracellular Vesicles·Federico CocozzaLorena Martin-Jaular
Oct 8, 2020·Canadian Journal of Microbiology·Arjun SukumaranJennifer Geddes-McAlister
Dec 29, 2020·International Journal of Nanomedicine·Xuelian FanGang Chen
Feb 9, 2021·Frontiers in Immunology·Dominique Missiakas, Volker Winstel
Dec 19, 2020·Frontiers in Oncology·Kaixiang FengDetao Yin
Jan 1, 2021·Frontiers in Pharmacology·Junghyun Lim, Aditya Murthy
Feb 4, 2021·International Journal of Nanomedicine·Dongki Yang
Feb 2, 2021·Frontiers in Microbiology·Caleb A FordJames E Cassat
Mar 9, 2021·Immunological Reviews·João FevereiroJorge Pedrosa
Mar 5, 2021·Frontiers in Immunology·Raquel Bragante GritteFrancisco Garcia Soriano
Feb 27, 2021·Journal of Molecular and Cellular Cardiology·Madeleine R MilesGea-Ny Tseng
Mar 9, 2021·Advanced Nanobiomed Research·Halle LutzKe Cheng
Apr 15, 2021·BMC Biology·Dustin A AmmendoliaJohn H Brumell
Apr 17, 2021·Biochemical Society Transactions·Joni Renee WhiteSimon Swift
Apr 23, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xingru YangBin Liu
Apr 22, 2020·Developmental Cell·Elena PanizzaMarc A Antonyak
Apr 28, 2021·Infectious Diseases·Ifeanyi Elibe MbaOnyekachi Philomena Okeke
May 18, 2021·Frontiers in Pharmacology·Peng QiuYang Liu
Jun 19, 2021·Advanced Drug Delivery Reviews·Crescenzo MassaroS Rubina Baglio
Jun 18, 2021·ACS Applied Materials & Interfaces·Divakara Ssm UppuYoon-Kyoung Cho
Aug 8, 2021·Nanomaterials·Syed Mohammed Basheeruddin AsdaqKatharigatta N Venugopala
Aug 24, 2021·Frontiers in Cardiovascular Medicine·Camila Rodrigues MorenoMaria de Lourdes Higuchi
Aug 31, 2021·The EMBO Journal·Daniel J KlionskyFederico Pietrocola
Oct 13, 2021·Trends in Immunology·Varnesh Tiku, Man-Wah Tan

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Methods Mentioned

BETA
flow-cytometry
flow cytometry
transmission electron microscopy
fluorescence-activated cell sorting
bronchoalveolar lavage
transfection
broncholear lavage
PCR

Software Mentioned

Sequest HT
Proteome Discoverer

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