Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders

Theriogenology
Joaquín GadeaSalvador Ruiz

Abstract

Although glutathione content in boar spermatozoa has been previously reported, the effect of reduced glutathione (GSH) on semen parameters and the fertilizing ability of boar spermatozoa after cryopreservation has never been evaluated. In this study, GSH content was determined in ejaculated boar spermatozoa before and after cryopreservation. Semen samples were centrifuged and GSH content in the resulting pellet monitored spectrophotometrically. The fertilizing ability of frozen-thawed boar sperm was also tested in vitro by incubating sperm with in vitro matured oocytes obtained from gilts. GSH content in fresh semen was 3.84 +/- 0.21 nM GSH/10(8) sperm. Following semen cryopreservation, there was a 32% decrease in GSH content (P < 0.0001). There were significant differences in sperm GSH content between different boars and after various preservation protocols (P = 0.0102 ). The effect of addition of GSH to the freezing and thawing extenders was also evaluated. Addition of 5 mM GSH to the freezing extender did not have a significant effect on standard semen parameters or sperm fertilizing ability after thawing. In contrast, when GSH was added to the thawing extender, a dose-dependent tendency to increase in sperm fertilizing abil...Continue Reading

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Citations

Feb 23, 2010·Asian Journal of Andrology·Juan Carlos Martinez-SotoJoaquín Gadea
Jul 28, 2009·Asian Journal of Andrology·Panida ChanapiwatPadet Tummaruk
Jul 6, 2010·Asian Journal of Andrology·Kampon KaeoketMongkol Techakumphu
Jun 23, 2012·PloS One·Mary HagedornStuart A Meyers
Sep 24, 2008·Fish Physiology and Biochemistry·Ping LiOtomar Linhart
Jan 26, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Xiao-Gang ZhangJian-Hong Hu
Jan 2, 2013·Cryobiology·Mohsen ForouzanfarMohammad Hossein Nasr Esfahani
Nov 3, 2012·Theriogenology·S A HussainM Anzar
Oct 2, 2012·Animal Reproduction Science·Luis Anel-LópezFelipe Martínez-Pastor
Jun 8, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Jan 3, 2016·Animal Reproduction Science·Hamed Mirzaei RadAbolfazl Ghanie
Dec 1, 2015·Polish Journal of Veterinary Sciences·M Trzcińska, M Bryła
Nov 11, 2015·Animal Reproduction Science·M A SilvestreJ L Yániz
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·J C MonteiroC I Vannucchi
May 20, 2008·Reproduction in Domestic Animals = Zuchthygiene·K BuranaamnuayM Techakumphu
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Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·M Yeste
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·P Sutovsky

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