Decrease in mitochondrial oxidative protein damage parameters in the streptozotocin-diabetic rat

Diabetes/metabolism Research and Reviews
R KayaliTuncay Altug

Abstract

Many authors have shown that hyperglycemia leads to an increase in oxidative protein damage in diabetes. The aim of this study was to reveal the susceptibility of mitochondria from liver, pancreas, kidney, and skeletal muscle of diabetic Sprague-Dawley rats, a model of type 1 diabetes, to oxidative protein damage. Mitochondrial fractions were obtained by differential centrifugation. To show the effect of hyperglycemia in promoting oxidative protein damage, we determined mitochondrial protein carbonyl, total thiol, nitrotyrosine, advanced oxidation protein products, and lipid hydroperoxide levels. The levels of the studied markers, except nitrotyrosine, were determined by colorimetric methods. Nitrotyrosine levels were measured by ELISA. All values were compared with those of the controls by using the Mann-Whitney U-test. Nitrotyrosine and lipid hydroperoxide levels were decreased and other parameters were not changed in liver mitochondria of diabetic rats. Protein carbonyl, nitrotyrosine, advanced oxidation protein products, and lipid hydroperoxide levels were decreased and total thiol levels were not changed in pancreas mitochondria of diabetic rats. Only advanced oxidation protein products and lipid hydroperoxide levels were ...Continue Reading

Citations

Apr 1, 2009·International Journal of Molecular Sciences·Emilie Chanséaume, Béatrice Morio
Oct 25, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kahkashan RashidParames C Sil
Apr 29, 2005·Diabetes/metabolism Research and Reviews
Aug 21, 2008·Sports Medicine·José A LuminiAntónio Ascensão

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