PMID: 19920389Nov 19, 2009Paper

Decrease in prostate specific antigen secretion correlated with docetaxel-induced growth inhibition and apoptosis in human prostate tumor cells

Gan to kagaku ryoho. Cancer & chemotherapy
Keiko NoguchiYasushi Yoshida

Abstract

The antitumor activity of docetaxel in human prostate tumor cells and correlation between cell-growth inhibition and prostate specific antigen (PSA) secretion were investigated. Cultured human prostate tumor cell lines (LNCaP, DU1 45 and PC-3) were treated with test drugs,after which the number of viable cells and PSA levels in the medium were determined. Apoptosis was assessed by changes in chromatin structure, DNA fragmentation, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) and Annexin V assay. Docetaxel inhibited cell proliferation in a dose-dependent fashion in all cell lines,with IC50 levels from 0.90 to 4.2 nM,which were similar to those of paclitaxel,but more potent than mitoxantrone,estramustine,or cisplatin. Docetaxel-treated cells underwent cell-cycle arrest in the G2/M phase and apoptosis as indicated by chromatin condensation and DNA fragmentation. In docetaxel-treated LNCaP cells,there was a linear correlation between growth inhibition and the decline in PSA level in the culture medium. It was demonstrated that docetaxel had potent antitumor activity against human prostate tumor cells,and the decrease in cell growth was associated with a decrease in PSA secretion,suggesting that PSA...Continue Reading

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis