Decrease of myofiber branching via muscle-specific expression of the olfactory receptor mOR23 in dystrophic muscle leads to protection against mechanical stress

Skeletal Muscle
Christophe PichavantGrace K Pavlath

Abstract

Abnormal branched myofibers within skeletal muscles are commonly found in diverse animal models of muscular dystrophy as well as in patients. Branched myofibers from dystrophic mice are more susceptible to break than unbranched myofibers suggesting that muscles containing a high percentage of these myofibers are more prone to injury. Previous studies showed ubiquitous over-expression of mouse olfactory receptor 23 (mOR23), a G protein-coupled receptor, in wild type mice decreased myofiber branching. Whether mOR23 over-expression specifically in skeletal muscle cells is sufficient to mitigate myofiber branching in dystrophic muscle is unknown. We created a novel transgenic mouse over-expressing mOR23 specifically in muscle cells and then bred with dystrophic (mdx) mice. Myofiber branching was analyzed in these two transgenic mice and membrane integrity was assessed by Evans blue dye fluorescence. mOR23 over-expression in muscle led to a decrease of myofiber branching after muscle regeneration in non-dystrophic mouse muscles and reduced the severity of myofiber branching in mdx mouse muscles. Muscles from mdx mouse over-expressing mOR23 significantly exhibited less damage to eccentric contractions than control mdx muscles. The de...Continue Reading

References

Dec 1, 1977·Journal of Neurology, Neurosurgery, and Psychiatry·M Swash, M S Schwartz
Jan 1, 1976·Tissue & Cell·H Schmalbruch
Feb 1, 1985·Journal of the Neurological Sciences·M SadehL Z Stern
Aug 1, 1971·The Anatomical Record·F P Moss, C P Leblond
May 1, 1981·Muscle & Nerve·M Ontell
Aug 1, 1993·Neuropathology and Applied Neurobiology·T A RobertsonM D Grounds
Nov 1, 1993·Journal of the Neurological Sciences·J K McGeachieJ E Morgan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 28, 2003·Biochemical and Biophysical Research Communications·Sayako KatadaKazushige Touhara
Oct 8, 2003·Acta Neuropathologica·Manuel RoigFrancina Munell
Dec 10, 2003·The Journal of Cell Biology·Bénédicte ChazaudRomain K Gherardi
Sep 23, 2006·Science·Takeshi ImaiHitoshi Sakano
Jun 15, 2007·American Journal of Physiology. Cell Physiology·S ChanJ W Morley
Feb 7, 2008·The Journal of Biological Chemistry·Joseph G ReynoldsFrancisco J Naya
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jennifer L PluznickMichael J Caplan
Apr 25, 2009·The Journal of Biological Chemistry·Eva M NeuhausHanns Hatt
Jul 17, 2009·American Journal of Physiology. Cell Physiology·Richard M LoveringChristopher W Ward
Nov 20, 2009·Developmental Cell·Christine A GriffinGrace K Pavlath
Dec 1, 2009·Lancet Neurology·Katharine BushbyUNKNOWN DMD Care Considerations Working Group
Mar 23, 2011·Experimental Physiology·S Chan, S I Head
Nov 29, 2012·BMB Reports·NaNa Kang, JaeHyung Koo
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jennifer L PluznickMichael J Caplan
May 24, 2014·Skeletal Muscle·Christophe Pichavant, Grace K Pavlath
Jun 8, 2014·Disease Models & Mechanisms·Chunhui JiangShihuan Kuang
Apr 25, 2015·Physiological Reports·Erick O Hernández-OchoaRichard M Lovering
Jul 4, 2015·Experimental Physiology·Simon GuiraudKay E Davies

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Citations

Nov 9, 2017·Skeletal Muscle·Abigail L Mackey, Michael Kjaer
Jan 15, 2019·Exercise and Sport Sciences Reviews·Kevin A MurachJohn J McCarthy
Apr 6, 2018·Genomics & Informatics·S June Oh
Dec 14, 2018·Frontiers in Physiology·Nicholas M DalesioDan E Berkowitz
Feb 20, 2021·Critical Reviews in Food Science and Nutrition·Rifat Nowshin RakaJianming Jin
Sep 4, 2020·Journal of Applied Physiology·Katherine E BukovecRobert W Grange

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