Decrease of Trefoil factor 2 in cats with feline idiopathic cystitis

BJU International
Stephanie I K LembergerCornelia A Deeg

Abstract

To obtain new insights into aetiological backgrounds, and to search for diagnostic biomarkers by assessing the difference in urinary proteins between cats with spontaneous feline idiopathic cystitis (FIC) and healthy controls. Urine supernatants of 18 cats with FIC and 18 healthy control cats, and bladder biopsies of two FIC diseased cats and four healthy controls were included in the study. The Bradford method was used to determine protein quantity in urine supernatants. Urine was separated by two-dimensional (2-D) gel electrophoresis. Selected protein spots were excised from two-dimensional gels and analysed with tandem mass spectrometry. Validation of Trefoil factor 2 expression was realized with Western blot and immunohistochemistry. Western blot signal intensities were quantified with image quant software. Eleven differentially expressed protein spots were identified between the 2-D gels of cats with FIC and control cats. Ten spots (only visible in the FIC gel) were identified as albumin and one spot (only visible in the control gel) was identified as Trefoil factor 2.Using quantification of Western blot signal intensities and immunohistochemistry a decrease in Trefoil factor 2 (TFF2) in cats with FIC could be revealed for...Continue Reading

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Citations

Feb 11, 2014·Genomics, Proteomics & Bioinformatics·Xundou LiYouhe Gao
Mar 31, 2015·The Veterinary Clinics of North America. Small Animal Practice·S Dru Forrester, Todd L Towell
May 29, 2015·Journal of Feline Medicine and Surgery·Heidi S LundAnna V Eggertsdóttir
Jun 27, 2018·Journal of Feline Medicine and Surgery·Heidi S Lund, Anna V Eggertsdóttir
Jan 23, 2018·Journal of Veterinary Internal Medicine·M ParysJ M Kruger
Jan 25, 2013·International Neurourology Journal·Sungyong YouJayoung Kim

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