Decreased acetylcholine receptor content in denervated skeletal muscles infused with nerve extract

Journal of Neuroscience Research
S T SayersI R Held

Abstract

The influence of a concentrated extract of soluble substances from the sciatic nerve upon the acetylcholine receptor (AChR) content in the soleus muscle of adult rats was examined by in vivo infusions. Internal and membrane-inserted AChR were quantitated by the specific binding of 125I-alpha-bungarotoxin (a-BuTX). Interestingly, the nerve extract had no apparent effect unless the soleus muscle was also denervated at the start of the infusion. Then, after 66 hr, substantially less (60-80%) binding of 125I-a-BuTX to AChR was observed compared to denervated solei that did not receive an infusion of nerve extract. However, the concentration of protein in the nerve extract had to exceed 5 mg/ml before this effect was evident. Infusions of phosphate-buffered saline, bovine serum albumin, rat liver extract, or human transferrin had no striking effect upon AChR. The prevention of the characteristic denervation-induced increase in non-junctional AChR by an active component in the nerve extract may be due to a trophic signal for decreased synthesis of AChR, but it is also possible that the degradation of AChR was increased.

References

Aug 1, 1978·The American Journal of Anatomy·Y C Wong, M C Ip
May 1, 1979·The American Journal of Physiology·J R Musick
Jan 1, 1979·Physiological Reviews·D M Fambrough
Dec 31, 1974·Biochemistry·R R AlmonS H Appel
Jan 1, 1983·Progress in Neurobiology·J J McArdle
Jul 1, 1984·The Journal of Cell Biology·J P MerlieJ R Sanes
Jan 1, 1981·Sub-cellular Biochemistry·S K Malhotra
Jan 1, 1982·Annual Review of Physiology·D W Pumplin, D M Fambrough
Jan 1, 1982·Journal of Neuroscience Research·T H Oh, G J Markelonis

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