Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system.

The EMBO Journal
Junyu ChenAnjali P Kusumbe

Abstract

Age-associated alterations of the hormone-secreting endocrine system cause organ dysfunction and disease states. However, the cell biology of endocrine tissue ageing remains poorly understood. Here, we perform comparative 3D imaging to understand age-related perturbations of the endothelial cell (EC) compartment in endocrine glands. Datasets of a wide range of markers highlight a decline in capillary and artery numbers, but not of perivascular cells in pancreas, testis and thyroid gland, with age in mice and humans. Further, angiogenesis and β-cell expansion in the pancreas are coupled by a distinct age-dependent subset of ECs. While this EC subpopulation supports pancreatic β cells, it declines during ageing concomitant with increased expression of the gap junction protein Gja1. EC-specific ablation of Gja1 restores β-cell expansion in the aged pancreas. These results provide a proof of concept for understanding age-related vascular changes and imply that therapeutic targeting of blood vessels may restore aged endocrine tissue function. This comprehensive data atlas offers over > 1,000 multicolour volumes for exploration and research in endocrinology, ageing, matrix and vascular biology.

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Citations

Mar 27, 2021·Frontiers in Physiology·Sina StuckerAnjali P Kusumbe
May 14, 2021·Frontiers in Endocrinology·Martha Campbell-Thompson, Shiue-Cheng Tang
May 14, 2021·Frontiers in Endocrinology·Guzel BurganovaLimor Landsman

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Methods Mentioned

BETA
PCR
flow cytometry
ELISA
ELISAs
transgenics
electron microscopy
transgenic
FACS

Software Mentioned

BD FACSDiva
Zeiss
Emcn
Imaris Distance Transformation XTensions
Zen Black
Crop 3D
Adobe Photoshop
Fiji
GraphPad Prism
Imaris

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