Decreased cardiac glutathione peroxidase levels and enhanced mandibular apoptosis in malformed embryos of diabetic rats.

Diabetes
Parri WentzelUlf J Eriksson

Abstract

To characterize normal and malformed embryos within the same litters from control and diabetic rats for expression of genes related to metabolism of reactive oxygen species (ROS) or glucose as well as developmental genes. Embryos from nondiabetic and streptozotocin-induced diabetic rats were collected on gestational day 11 and evaluated for gene expression (PCR) and distribution of activated caspase-3 and glutathione peroxidase (Gpx)-1 by immunohistochemistry. Maternal diabetes (MD group) caused growth retardation and an increased malformation rate in the embryos of MD group rats compared with those of controls (N group). We found decreased gene expression of Gpx-1 and increased expression of vascular endothelial growth factor-A (Vegf-A) in malformed embryos of diabetic rats (MDm group) compared with nonmalformed littermates (MDn group). Alterations of messenger RNA levels of other genes were similar in MDm and MDn embryos. Thus, expression of copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), and sonic hedgehog homolog (Shh) were decreased, and bone morphogenetic protein-4 (Bmp-4) was increased, in the MD embryos compared with the N embryos. In MDm embryos, we detected increased activated caspa...Continue Reading

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Citations

Apr 1, 2009·Apoptosis : an International Journal on Programmed Cell Death·James H ChappellMary R Loeken
Mar 14, 2012·Cellular and Molecular Neurobiology·María Sol KruseHéctor Coirini
Oct 12, 2012·In Vitro Cellular & Developmental Biology. Animal·Gladys Chirino-GalindoMartín Palomar-Morales
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Methods Mentioned

BETA
glycosylation
PCR

Software Mentioned

Primer3
Statview

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