Decreased ceramide transport protein (CERT) function alters sphingomyelin production following UVB irradiation.

The Journal of Biological Chemistry
Alexandra CharruyerYoshikazu Uchida

Abstract

Increased cellular ceramide accounts in part for UVB irradiation-induced apoptosis in cultured human keratinocytes with concurrent increased glucosylceramide but not sphingomyelin generation in these cells. Given that conversion of ceramide to non-apoptotic metabolites such as sphingomyelin and glucosylceramide protects cells from ceramide-induced apoptosis, we hypothesized that failed up-regulation of sphingomyelin generation contributes to ceramide accumulation following UVB irradiation. Because both sphingomyelin synthase and glucosylceramide synthase activities were significantly decreased in UVB-irradiated keratinocytes, we investigated whether alteration(s) in the function of ceramide transport protein (or CERT) required for sphingomyelin synthesis occur(s) in UVB-irradiated cells. Fluorescently labeled N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-d-erythro-sphingosine (C(5)-DMB-ceramide) relocation to the Golgi was diminished after irradiation, consistent with decreased CERT function, whereas the CERT inhibitor N-(3-hydroxy-1-hydroxymethyl-3-phenylpropyl)dodecanamide (1R,3R isomer) (HPA-12) produced an equivalent effect. UVB irradiation also induced the rapid formation of a stable CERT homotrim...Continue Reading

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Citations

Dec 12, 2012·Molecular and Cellular Biology·Kyungho ParkYoshikazu Uchida
Aug 31, 2013·Biochimica Et Biophysica Acta·Mariona RabionetRoger Sandhoff
Sep 24, 2013·Biochimica Et Biophysica Acta·Yoshikazu Uchida
Jan 10, 2012·Biochimica Et Biophysica Acta·Monilola A Olayioye, Angelika Hausser
Dec 3, 2011·Progress in Lipid Research·Sabine GröschGerd Geisslinger
Jul 17, 2013·The Journal of Investigative Dermatology·Kyungho ParkYoshikazu Uchida
Jun 4, 2010·The Journal of Investigative Dermatology·Yoshikazu UchidaWalter M Holleran
Apr 15, 2011·Journal of Lipids·Wei-Ching HuangChiou-Feng Lin
Feb 27, 2015·Journal of Biomolecular Screening·Laurence FleuryFrédéric Ausseil
Feb 27, 2017·Bioorganic & Medicinal Chemistry·Cécile SantosYves Génisson
Jun 4, 2014·Chemistry, an Asian Journal·Essa M SaiedChristoph Arenz
Sep 16, 2016·Chemistry : a European Journal·Dušan BerkešYves Génisson
Sep 27, 2014·Chembiochem : a European Journal of Chemical Biology·Cécile SantosYves Génisson
Dec 18, 2020·Frontiers in Molecular Biosciences·Mariem GhoulaOlivier Taboureau

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