PMID: 9446853Mar 7, 1998Paper

Decreased citrate improves iron availability from infant formula: application of an in vitro digestion/Caco-2 cell culture model

The Journal of Nutrition
R P GlahnD R Van Campen

Abstract

We have applied an in vitro digestion/Caco-2 cell culture model to the assessment of iron availability from human milk and a generic cow's milk-based infant formula. Experiments were designed to determine the availability of iron from human milk relative to infant formula and whether known promoters of iron absorption would increase Caco-2 cell iron uptake and availability from the infant formula. In addition, we sought to determine if decreasing the citrate concentration in the infant formula would increase the iron uptake. Although approximately twice as much iron was in solution from digests of the infant formula relative to that of human milk, smaller or equal amounts of iron were taken up from the infant formula relative to the human milk digest. These results are qualitatively similar to in vivo studies. Addition of known iron uptake promoters to infant formula did not enhance Caco-2 cell iron uptake from the infant formula digest, indicating that the iron in the infant formula existed predominantly in a tightly bound unavailable form(s). Enzymatic pretreatment of the infant formula with citrate lyase and oxalacetate decarboxylase decreased the citrate concentration by 67% and resulted in a 64% increase of iron in solutio...Continue Reading

References

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Citations

Mar 25, 2000·The American Journal of Clinical Nutrition·M B ReddyJ D Cook
Jan 30, 2004·The Journal of Nutrition·Dora Inés MazariegosMiguel Arredondo
Sep 3, 2008·Journal of Zhejiang University. Science. B·Wan-ling HeXiao-e Yang
Feb 5, 2003·The Journal of Nutrition·Robert E Serfass, Manju B Reddy
Oct 6, 2016·Journal of the Science of Food and Agriculture·Bahareh Nikooyeh, Tirang R Neyestani
Jul 1, 2004·The Journal of Nutrition·Eun Chul HuhRaymond P Glahn
May 3, 2007·Journal of Medicinal Food·Maia RabinovichNéstor Curvetto
Feb 6, 2010·Journal of Medicinal Food·Débora FiglasNéstor Curvetto
May 19, 2018·Medicines·Gabriela M ChiocchettiTatiana Christides
Feb 18, 2011·European Journal of Nutrition·Pía VillarroelMiguel Arredondo
Jan 7, 2014·Annual Review of Food Science and Technology·Gail M Bornhorst, R Paul Singh
Jan 29, 2021·Critical Reviews in Food Science and Nutrition·Yaxing ManBo Wang
Sep 2, 2003·The Journal of Nutrition·Sean R Lynch, Rebecca J Stoltzfus
Mar 18, 2009·Journal of Agricultural and Food Chemistry·Indah EpriliatiMike Gidley
Oct 29, 2013·Journal of Agricultural and Food Chemistry·Ravindranadh PalikaPullakhandam Raghu
Aug 21, 2007·Journal of Agricultural and Food Chemistry·Magnolia Ariza-NietoRaymond P Glahn
Mar 27, 2010·Neurochemistry International·Belinda M KeenanGlenda M Bishop

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