Jan 1, 1989

Decreased content of the 35 kDa cytoskeletal protein p35 in Friend erythroleukemia cells exposed to dimethyl sulfoxide and retinoic acid is associated with entrance into a quiescent substrate

The International Journal of Biochemistry
P J HigginsF Traganos


1. The effect of all-trans-retinoic acid (RA) on cell cycle kinetics, RNA content, and expression of the 35 kDa cytoskeletal protein p35 in exponentially-growing Friend erythroleukemia (FL) cells was compared with the prototypic differentiation-inducer dimethylsulfoxide (DMSO). 2. Two G1 phase populations of RA-treated FL cells were identified: one with an intermediate RNA content (T-cells) similar to G1 cells in near-plateau-phase control cultures and the other with a very low RNA content (Q-cells) similar to DMSO-differentiated cells; although quiescent, RA-treated cells remained undifferentiated as evidenced by the absence of late-stage markers of erythroid maturation. 3. Decreases in the cellular content of p35 occurred in both DMSO- and RA-treated FL cells, correlating with the onset of accumulation of cells into G1, and stabilized by 48 hr after initial exposure to either inducer. 4. Down-regulation in the cellular p35 content, thus, appears to be linked to entrance of FL cells into a quiescent substrate and independent of the subsequent capacity for erythroid differentiation.

  • References14
  • Citations1
  • References14
  • Citations1


Mentioned in this Paper

Dimethyl Sulfoxide
Biologic Development
DNA, Neoplasm
Cell Differentiation Process
Cell Cycle
Staining and Labeling
Cytoskeletal Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.