Decreased coronary arteriolar response to KCa channel opener after cardioplegic arrest in diabetic patients.

Molecular and Cellular Biochemistry
Yuhong LiuJun Feng

Abstract

We have recently found that diabetes is associated with the inactivation of the calcium-activated potassium channels (KCa) in endothelial cells, which may contribute to endothelial dysfunction in diabetic patients at baseline. In the current study, we further investigated the effects of diabetes on coronary arteriolar responses to the small (SK) and intermediate (IK) KCa opener NS309 in diabetic and non-diabetic patients and correlated that data with the changes in the SK/IK protein expression/distribution in the setting of cardioplegic ischemia and reperfusion (CP) and cardiopulmonary bypass (CPB). Coronary arterioles from the harvested right atrial tissue samples from diabetic and non-diabetic patients (n = 8/group) undergoing cardiac surgery were dissected pre- and post-CP/CPB. The in vitro relaxation response of pre-contracted arterioles was examined in the presence of the selective SK/IK opener NS309 (10-9-10-5 M). The protein expression/localization of KCa channels in the harvested atrial tissue samples, coronary microvessels, and primary cultured human coronary endothelial cells were assayed by Western blotting and immunohistochemistry. The relaxation response to NS309 post-CP/CPB was significantly decreased in diabetic ...Continue Reading

References

Nov 3, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Marc RuelFrank W Sellke
Oct 19, 2005·British Journal of Pharmacology·Hong DingChris Triggle
May 10, 2006·British Journal of Pharmacology·Matthew P BurnhamArthur H Weston
Feb 27, 2010·Reviews in Endocrine & Metabolic Disorders·Corey E TabitJoseph A Vita
Sep 2, 2010·The Journal of Thoracic and Cardiovascular Surgery·Michael P RobichFrank W Sellke
Jun 13, 2015·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Jun FengFrank W Sellke

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Citations

Aug 3, 2019·International Journal of Molecular Sciences·Kenichi Goto, Takanari Kitazono
Mar 23, 2020·International Journal of Cardiology·Yuhong LiuJun Feng

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