Decreased cytochrome c mediates an age-related decline of oxidative phosphorylation in rat kidney mitochondria.

The Biochemical Journal
John F O'TooleC L Hoppel

Abstract

Kidney function declines with advancing age and mitochondria have been implicated. In the present study we have examined the integrated function of mitochondria isolated from kidneys of 6- and 24-month-old Fischer 344 rats. OXPHOS (oxidative phosphorylation) of intact mitochondria and cytochrome c oxidase activity in permeabilized mitochondria were determined with polarographic assays. The activities of the ETC (electron transport chain) complexes and the cytochrome content in solubilized mitochondria were measured using spectrophotometric methods. The respiratory complexes were evaluated with blue native gel electrophoresis. Mitochondrial preparations were evaluated by immunoblotting for cytochrome c, Smac/Diablo and VDAC (voltage-dependent anion channel). Mitochondrial morphology was examined by electron microscopy. OXPHOS of mitochondria isolated from 24-month-old animals was decreased 15-25% with complexes I, II, III and IV, and fatty acid substrates. The electron microscopic appearance of mitochondria, the activity of the ETC complexes and the protein abundance of individual complexes and supercomplexes were unchanged. The content of cytochrome c was decreased by 37% in aged mitochondria, as determined by spectrophotometri...Continue Reading

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Citations

Oct 20, 2012·Journal of Bioenergetics and Biomembranes·Janet E SaundersRick G Schnellmann
Jul 26, 2013·Environmental Science & Technology·Yan ZhangHongqiang Ren
Apr 6, 2012·Expert Opinion on Drug Metabolism & Toxicology·David M Small, Glenda C Gobe
Oct 13, 2011·BioFactors·Maria Luisa Genova, Giorgio Lenaz
Apr 24, 2016·Free Radical Biology & Medicine·Raquel Moreno-Loshuertos, José Antonio Enríquez
Jun 3, 2016·International Journal of Cardiology·Tomasz A BondaKarol A Kamiński
Dec 24, 2010·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Vijay VenugopalRobert M Levin
Sep 29, 2017·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Larisa EmelyanovaArshad Jahangir
Jun 27, 2018·Current Protocols in Cytometry·Joseph R DanieleAndrew Dillin
Nov 2, 2016·Menopause : the Journal of the North American Menopause Society·William J LawsonBernadette E Grayson
Aug 1, 2018·Frontiers in Aging Neuroscience·Alireza MajdiAlbert Gjedde
Nov 28, 2017·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tomasz A BondaKarol A Kamiński
Oct 18, 2020·International Journal of Molecular Sciences·Michael Webb, Dionisia P Sideris
Nov 18, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sabzali JavadovAmadou Ks Camara
Apr 18, 2021·Archives of Environmental Contamination and Toxicology·Sevgi Başalan ÖverYusuf Sevgiler

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