Decreased expression of heat shock proteins may lead to compromised wound healing in type 2 diabetes mellitus patients

Journal of Diabetes and Its Complications
Kanhaiya SinghKiran Singh

Abstract

Heat shock proteins (HSPs) are inducible stress proteins expressed in cells exposed to stress. HSPs promote wound healing by recruitment of dermal fibroblasts to the site of injury and bring about protein homeostasis. Diabetic wounds are hard to heal and inadequate HSPs may be important contributors in the etiology of diabetic foot ulcers (DFU). To analyze the differential expression of HSPs and their downstream molecules in human diabetic wounds compared to control wounds. Expressional levels of HSP27, HSP47 and HSP70 and their downstream molecules like TLR4, p38-MAPK were seen in biopsies from 101 human diabetic wounds compared to 8 control subjects without diabetes using RT-PCR, western blot and immunohistochemistry. Our study suggested a significant down regulation of HSP70, HSP47 and HSP27 (p value=<0.001 for HSP70; p value=0.007 for HSP47; p value=0.007 for HSP27) in DFU along with their downstream molecules TLR4 and p38-MAPK (p value=0.006 for p38-MAPK; p value=0.02 for TLR4). HSP70 levels were significantly lower in male subjects and their levels increased significantly with the grades of wound on Wagner's scale. Infection status of the wounds was found to be significantly associated with the increased levels of HSP70 a...Continue Reading

References

Mar 26, 1998·Biochemical and Biophysical Research Communications·A ShuklaB S Srivastava
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A F LaplanteL Germain
Feb 6, 1999·The Journal of General Virology·E OhgitaniJ Imanishi
Aug 24, 1999·The Journal of Surgical Research·A L McMurtryD G Greenhalgh
Dec 22, 1999·Seminars in Cell & Developmental Biology·S R Lamandé, J F Bateman
Oct 11, 2001·The Journal of Biological Chemistry·T R DaffornA D Miller
Feb 14, 2002·The Journal of Surgical Research·Sahoko HiranoRobert R Gilmont
Feb 12, 2002·The Journal of Biological Chemistry·Alexzander AseaStuart K Calderwood
Jul 10, 2002·International Journal of Oral and Maxillofacial Surgery·Z L WangT Taguchi
Apr 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M R VossA A Knowlton
Jul 24, 2004·Cell Stress & Chaperones·Sahoko HiranoRobert R Gilmont
Mar 2, 2005·Diabetes Technology & Therapeutics·Philip L Hooper, Joanna J Hooper
Jul 31, 2007·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·M J D WagstaffD S Latchman
Nov 6, 2007·Annals of the New York Academy of Sciences·Stuart K CalderwoodPhillip J Gray
Jul 30, 2008·Cell Stress & Chaperones·Harm H KampingaLawrence E Hightower
Mar 12, 2009·Current Protein & Peptide Science·Mustafa AtalaySashwati Roy
Mar 5, 2010·Metabolism: Clinical and Experimental·Darren C HenstridgeBarbora de Courten
Oct 11, 2011·Biochimica Et Biophysica Acta·Wei LiFred Tsen
May 10, 2012·Neurobiology of Disease·L KorngutD W Zochodne
Sep 7, 2012·The Journal of Investigative Dermatology·Lin ChenLuisa A DiPietro
Oct 22, 2013·PloS One·Jonathan CroweJonathan L E Dean
Feb 14, 2014·Cell Stress & Chaperones·Philip L HooperLaszlo Vigh
Apr 20, 2014·The International Journal of Lower Extremity Wounds·Kanhaiya SinghKiran Singh

❮ Previous
Next ❯

Citations

Apr 3, 2019·Reviews in Endocrine & Metabolic Disorders·Mohammad Zubair, Jamal Ahmad
Dec 15, 2017·International Journal of Molecular Sciences·Stefania BelliniGabriella Gruden
Dec 3, 2020·International Journal of Nanomedicine·Karthik GourishettiRekha Raghuveer Shenoy
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Satish PatelDeependra Singh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis