Decreased Expression of TGR5 in Vogt-Koyanagi-Harada (VKH) Disease

Ocular Immunology and Inflammation
Jinglu YangHong Li

Abstract

Purpose: To investigate the role of G-protein-coupled bile acid receptor-1, Gpbar1 (TGR5) in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease.Methods: The mRNA level of TGR5, iNOS, Arg1, CD16, and CD206 in macrophages was assayed by real-time PCR. ELISA was used to detect the production of cytokines in cell culture supernatants. The frequencies of CD4+IFN-γ+ and CD4+ IL-17+ T cells were tested by flow cytometry.Results: A decreased expression of TGR5 in M1 macrophages was observed in active VKH patients as compared with normal controls. TGR5 stimulation of M1 macrophages with INT-777 caused a shift of the inflammatory M1 toward the anti-inflammatory M2 macrophage subtype. TGR5 activation of macrophages co-cultured with CD4+ T cells inhibited Th1 and Th17 polarization, as well as the release of IFN-γ and IL-17 in the culture supernatant.Conclusion: Our results show that a decreased TGR5 expression might contribute to the pathogenesis of VKH disease.

References

Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jan 14, 2003·The Journal of Biological Chemistry·Yuji KawamataMasahiko Fujino
Jun 5, 2003·Ocular Immunology and Inflammation·Kouichi Ohta, Nagahisa Yoshimura
Jun 26, 2004·Journal of Leukocyte Biology·Robert D Stout, Jill Suttles
Nov 25, 2006·Ophthalmology·Peizeng YangAize Kijlstra
Mar 6, 2007·The Journal of Allergy and Clinical Immunology·Wei ChiAize Kijlstra
Sep 11, 2007·Biochemical and Biophysical Research Communications·Hiroyuki SatoRégis Saladin
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Oct 19, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna BaghdasaryanMichael Trauner
Dec 14, 2011·Cell Metabolism·Thijs W H PolsKristina Schoonjans
Apr 23, 2013·Cell Metabolism·Thomas Q de Aguiar VallimPeter A Edwards
Aug 29, 2013·Nature Reviews. Gastroenterology & Hepatology·Frank G SchaapPeter L M Jansen
Jan 15, 2014·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Henri DubocAlan F Hofmann
May 14, 2014·Nature Reviews. Endocrinology·Folkert KuipersAlbert K Groen
Jul 31, 2014·Pharmacological Reviews·Tiangang Li, John Y L Chiang
Aug 7, 2014·Journal of Ophthalmic Inflammation and Infection·Joanne Yw NgChi-Pui Pang
Apr 1, 2015·Autoimmunity·David W MeltonPaula K Shireman
Sep 4, 2015·British Journal of Pharmacology·Man K S LeeJaye P F Chin-Dusting
Feb 16, 2016·Progress in Retinal and Eye Research·Liping DuPeizeng Yang
Jan 14, 2017·Frontiers in Physiology·Cong GuoYan-Dong Wang
Mar 16, 2017·The Journal of Cell Biology·Filipa B LopesDaniel M Davis
Jun 14, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michele BiagioliStefano Fiorucci
Apr 7, 2018·Investigative Ophthalmology & Visual Science·Xinyue HuangPeizeng Yang

❮ Previous
Next ❯

Citations

Jan 1, 2021·Journal of Immunology Research·Jun-Bin YanBei-Hui He

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Verena KeitelDieter Häussinger
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Wei-Dong ChenYan-Dong Wang
Diagnostic Pathology
Chaohui Lisa ZhaoWeibiao Cao
© 2022 Meta ULC. All rights reserved