PMID: 7009651Apr 1, 1981Paper

Decreased in vitro humoral immune responses in aged humans

The Journal of Clinical Investigation
S G PahwaR A Good

Abstract

Induction of antigen-specific and non-specific (polyclonal) humoral immune responses in vitro was investigated in peripheral blood mononuclear cells of aged (65-85 yr) and young (20-30 yr) volunteers. In vitro immunization of lymphocytes with antigen (sheep erythrocytes) was performed in a recently described microculture system, and anti-sheep erythrocyte plaque forming cells were quantitated in a direct hemolytic plaque assay. Immunoglobulin secreting cells, induced polyclonally with pokeweed mitogen, were quantitated in a reverse hemolytic plaque assay. Significant depressions of antigen-specific as well as polyclonal responses were noted in relation to advancing age. Antigen-specific responses were more frequently depressed than polyclonal responses. T cell mitogen concanavalin A (Con A) was used to amplify functions of autologous immunoregulatory T cells. Addition of 10 microgram/ml Con A to lymphocytes of young donors at culture initiation resulted in activation of suppressor cells and abrogated antigen-specific responses. Delayed addition of Con A, on the other hand, enhanced responses, presumably because of activation of helper T cells. Similar manipulations of lymphocyte cultures from aged donors showed failure of Con A...Continue Reading

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Citations

Dec 30, 1982·Journal of Immunological Methods·M K HoffmannR S Mittler
Sep 1, 1983·Mechanisms of Ageing and Development·J P AntelJ E Hopper
May 31, 1985·Mechanisms of Ageing and Development·S AntonaciL Bonomo
Nov 1, 1987·Mechanisms of Ageing and Development·S AntonaciL Bonomo
Sep 1, 1988·Mechanisms of Ageing and Development·D R Davila, K W Kelley
Nov 1, 1992·Mechanisms of Ageing and Development·M J Steffen, J L Ebersole
Jun 1, 1993·Mechanisms of Ageing and Development·C TortorellaS Antonaci
Sep 15, 2001·Mechanisms of Ageing and Development·K WatanabeY Iwatani
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S G PahwaR A Good
Oct 1, 1983·Journal of Clinical Immunology·E De Bernardo, T F Davies
Jan 1, 1991·Immunopharmacology and Immunotoxicology·S AntonaciL Bonomo
Dec 1, 1983·Journal of the American Geriatrics Society·J M Felser, M J Raff
Feb 1, 1990·Oral Surgery, Oral Medicine, and Oral Pathology·A H Friedlander, T T Yoshikawa
Dec 20, 2000·The Journal of Infection·R J DobbsC Weller
Dec 1, 1989·Journal of the American Geriatrics Society·J CrawfordH J Cohen
Sep 1, 1987·European Journal of Immunology·H Kawanishi, J Kiely
May 3, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suresh PallikkuthSavita Pahwa
Jan 1, 1988·Autoimmunity·Y Tomer, Y Shoenfeld

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