PMID: 6991325Mar 1, 1980Paper

Decreased insulin binding and degradation associated with depressed intracellular ATP content

Diabetes
B DrazninK E Sussman

Abstract

The effect of metabolic inhibitors, 2,4-dinitrophenol (DNP) and NaF, on insulin binding and degradation has been studied in cultured Buffalo rat liver (BRL) cells. In control studies, 1.8 fmol of 125I-insulin binds to 1.2 x 10(6) cells, possessing approximately 40,000 receptor sites per cell with binding affinity of 5.52 x 10(-8) M. When the cells were preincubated with increasing concentrations of either DNP or NaF, a dose- and time-dependent decrease in both insulin binding and degradation was observed. The total amount of 125I-insulin bound to BRL cells preincubated with metabolic inhibitors was reduced to 1.2 fmol per 1.2 x 10(6) cells. The point of 1/2 B max was achieved in the presence of 50 ng/ml of native insulin, 1.7 times that of the control level. The number of receptor sites was unaffected by either DNP or NaF, but an average affinity profile revealed a decrease in the affinity of the ATP-depleted cells for insulin (KD: 7.31 x 10(-8) M and 7.06 x 10(-8) M in DNP- and NaF-treated cells, respectively). The decrease in insulin binding and degradation following the exposure of the BRL cells to the metabolic inhibitors was associated with a 20% reduction in intracellular ATP and adenylate energy charge. DNP and NaF did n...Continue Reading

Citations

Dec 1, 1981·Digestive Diseases and Sciences·N SatoB Hagihara
Jul 1, 1985·The American Journal of the Medical Sciences·J M Yarborough
Jul 1, 1984·Archives of Biochemistry and Biophysics·J H ImH D Kim
Sep 1, 1984·Acta paediatrica Scandinavica·H KashiwaR Eto
Jun 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KiuchiR Pichlmayr
Dec 1, 1981·Acta Anaesthesiologica Scandinavica·J A Aldrete
May 23, 2001·Experimental Biology and Medicine·M C CamberosJ C Cresto

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