PMID: 32696744Jul 23, 2020Paper

Decreased long-chain non-coding RNA MALAT1 expression and increased hsa-miR155-3p expression involved in Notch signaling pathway regulation in rheumatoid arthritis patients

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Lei WanLi Zheng

Abstract

Objective To observe the expression of long-chain non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and hsa-miR155-3p in patients with rheumatoid arthritis (RA) and their relationship with Notch signaling pathway, and to explore the possible pathogenesis of RA. Methods Peripheral blood of RA patients (RA group) and healthy controls (NC group) were used to screen differentially expressed lncRNA and mRNA by high-throughput gene sequencing. Reverse-transcription PCR was used to detect the expression of lncRNA MALAT1, hsa-miR155-3p and Notch signaling pathway receptor ligands. Results The lncRNA sequencing analysis showed a total of 9158 differentially expressed lncRNAs. Gene ontology (GO) functional classification annotations revealed that the differentially expressed mRNA was mainly involved in immune inflammatory response, cellular transcriptional regulation and so on. Pathway analysis proved that differentially expressed mRNA was significantly related to the genes involved in rheumatoid arthritis, cellular senescence, and Notch signaling pathways. According to cis and trans prediction, Jagged1-2, Delta1-2 and Notch1-2 might be closely related to RA. MALAT1 in the RA group was lower than tha...Continue Reading

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