Decreased MHC I expression in IFN γ mutant mice alters synaptic elimination in the spinal cord after peripheral injury.

Journal of Neuroinflammation
Sheila C S VictórioAlexandre L R Oliveira

Abstract

The histocompatibility complex (MHC) class I expression in the central nervous system (CNS) regulates synaptic plasticity events during development and adult life. Its upregulation may be associated with events such as axotomy, cytokine exposition and changes in neuron electrical activity. Since IFNγ is a potent inducer of the MHC I expression, the present work investigated the importance of this pro-inflammatory cytokine in the synaptic elimination process in the spinal cord, as well as the motor recovery of IFN⁻/⁻, following peripheral injury. The lumbar spinal cords of C57BL/6J (wild type) and IFNγ⁻/⁻ (mutant) mice, subjected to unilateral sciatic nerve transection, were removed and processed for immunohistochemistry and real time RT-PCR, while the sciatic nerves from animals subjected to unilateral crush, were submitted to immunohistochemistry and electron microscopy for counting of the axons. Gait recovery was monitored using the Cat Walk system. Newborn mice astrocyte primary cultures were established in order to study the astrocytic respose in the absence of the IFNγ expression. IFNγ⁻/⁻ mutant mice showed a decreased expression of MHC I and β2-microglobulin mRNA coupled with reduced synaptophysin immunolabelling in the l...Continue Reading

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Citations

Jul 15, 2015·Experimental Brain Research·Aifeng ZhangJianqiong Zhang
Dec 3, 2014·Neuropharmacology·Aline Barroso Spejo, Alexandre L R Oliveira
Dec 29, 2017·The Journal of Experimental Medicine·Guodong SunZhinan Yin
Nov 20, 2016·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Daniel S TyleeStephen J Glatt

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Methods Mentioned

BETA
electron microscopy
PCR
Transmission electron microscopy

Software Mentioned

IMAGEJ
Graphpad Prisma
Cat Walk
ImageTool

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