Decreased MiR-128-3p alleviates the progression of rheumatoid arthritis by up-regulating the expression of TNFAIP3

Bioscience Reports
Zhongbin XiaGuoqing Li

Abstract

Background: Rheumatoid arthritis (RA) is a inflammatory disease that characterized with the destruction of synovial joint, which could induce disability. Inflammatory response mediated the RA. It has been reported that MiR-128-3p is significantly increased in RA, while the potential role was still unclear.Methods: T cells in peripheral blood mononuclear cell (PBMC) were isolated from the peripheral blood from people of RA and normal person were used. Real-time PCR was performed to detect the expression of MiR-128-3p, while the protein expression of tumor necrosis factor-α-induced protein 3 (TNFAIP3) was determined using Western blot. The levels of IL-6 and IL-17 were measured using enzyme-linked immunosorbent assay (ELISA). The expression of CD69 and CD25 was detected using flow cytometry. The RA mouse model was constructed for verification of the role of MiR-128-3p.Results: The expression of MiR-128-3p was significantly increased, while TNFAIP3 was decreased, the levels of IL-6 and IL-17 were also increased in the T cells of RA patients. Down-regulated MiR-128-3p significantly suppressed the expression of p-IkBα and CD69, and CD25in T cells. MiR-128-3p targets TNFAIP3 to regulate its expression. MiR-128-3p knockdown significan...Continue Reading

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Citations

Aug 5, 2020·International Journal of Rheumatic Diseases·Jijun CaoTingwang Jiang
Mar 14, 2019·International Journal of Molecular Sciences·Rüdiger Hardeland
Apr 30, 2020·Journal of Clinical Medicine·Daniel P ZalewskiAnna Bogucka-Kocka
Feb 22, 2021·Molecular and Cellular Biochemistry·Tao PengYankai Jiang
May 28, 2021·The Kaohsiung Journal of Medical Sciences·Ling-Ling Huo, Zhao-Rui Sun

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Methods Mentioned

BETA
flow cytometry
fluorescence activated cell storing
PCR
Assay
ELISA

Software Mentioned

TargetScan
Online

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