Decreased mRNA expression of several basement membrane components in basal cell carcinoma

The Journal of Investigative Dermatology
A ChopraN J Korman

Abstract

The biologic factors that control the behavior of basal cell carcinoma are poorly understood. This study was undertaken to elucidate the mechanisms responsible for the altered protein levels of several basement membrane components found in basal cell carcinoma. RNA was isolated from papulonodular basal cell carcinoma, normal human epidermal keratinocytes, and normal human skin, reverse transcribed to cDNA and amplified by the polymerase chain reaction utilizing primers specific for the 230 kDa bullous pemphigoid antigen (BPAG1), the 180 kDa bullous pemphigoid antigen (BPAG2), the alpha6 and beta4 chains of the alpha6beta4 integrin complex, and the beta3 chain of laminin 5. Southern blots probed with internal oligonucleotides confirmed that each polymerase chain reaction was specific for the basement membrane component amplified. The mRNA expressions of basement membrane components were indistinguishable between normal human epidermal keratinocytes and normal human skin, and subsequent experiments used normal human epidermal keratinocytes as controls. Quantitation of polymerase chain reaction products indicated that all basement membrane specific mRNA were significantly decreased in basal cell carcinoma as compared with normal h...Continue Reading

References

Jan 1, 1979·International Review of Cytology·N A KefalidesC C Clark
Sep 1, 1992·The Journal of Investigative Dermatology·G J GiudiceL A Diaz
Jan 11, 1992·The Journal of Investigative Dermatology·M M BryskS Rajaraman
Oct 1, 1992·The Journal of Investigative Dermatology·M Ståhle-BäckdahlW C Parks
Dec 1, 1990·Cancer Metastasis Reviews·W G Stetler-Stevenson
Jan 1, 1991·Journal of the American Academy of Dermatology·S J Miller
Jul 1, 1991·Archives of Dermatology·P A Ledwig, A S Paller
Dec 1, 1985·The Journal of Investigative Dermatology·J R StanleyV Klaus-Kovtun
Mar 1, 1987·The Journal of Investigative Dermatology·S H BarskyS Bhuta
Jul 1, 1985·The Journal of Investigative Dermatology·M OguchiG Asboe-Hansen
Jun 1, 1982·The Journal of Investigative Dermatology·J R StanleyS I Katz
Sep 1, 1995·The British Journal of Dermatology·J A FairleyG J Giudice
Mar 1, 1996·The Journal of Investigative Dermatology·D S WalshM Peacocke

❮ Previous
Next ❯

Citations

Nov 19, 2004·Neoplasia : an International Journal for Oncology Research·Robert CalaluceRay B Nagle
May 12, 2001·The Journal of Investigative Dermatology·J M BonifasE H Epstein
Jun 18, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mataleena ParikkaTuula Salo
Oct 31, 2000·Microscopy Research and Technique·D AberdamP Simon-Assmann
Nov 25, 2016·American Journal of Physiology. Cell Physiology·Mark A ChapmanRichard L Lieber
Mar 10, 2001·Molecular Carcinogenesis·R CalaluceR B Nagle
Jul 21, 2009·Journal of Cutaneous Pathology·Wedad Z MostafaEman El-Nabarawy
Mar 14, 2000·The Journal of Biological Chemistry·K A MillerS A Weitzman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Bullous Pemphigoid

Bullous pemphigoid is a rare immune system disorder and skin condition that causes large, fluid-filled blisters. Bullous pemphigoid commonly affects older adults and appears in areas such as the lower abdomen, upper thighs or armpits. Discover the latest research on bullous pemphigoid here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Carcinoma, Basal Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.