PMID: 7026617Oct 1, 1981Paper

Decreased sensitivity of old and progeric human fibroblasts to a preparation of factors with insulinlike activity

The Journal of Clinical Investigation
C B HarleyH Guyda

Abstract

To determine whether old cells have a reduced response to a preparation of factors from human plasma with insulinlike activity (ILA), we analyzed the response to ILA of early and late passage human fibroblasts from young, old, and progeric donors in the acute stimulation of [3H]2-deoxy-D-glucose (2dG) uptake and the delayed stimulation of [3H]thymidine (TdR) incorporation into DNA. The ILA concentration required to produce equivalent, relative stimulation of TdR incorporation was increased two- to three-fold in late passage cells and cells from old and progeric donors (P less than 0.01). 50 and 95% of maximal stimulation (ILA50, ILA95) was achieved by 0.26 +/- 0.07 and 1.38 +/- 0.13 ng insulin equivalents/ml (mean +/- SD) respectively, in cells from young adults at early passage. Corresponding values were 0.54 +/- 0.05 and 2.90 +/- 0.25 in cells from old donors; greater than 0.9 +/- 0.1 and greater than 3.1 +/- 0.1 in cells from a 9-yr-old progeric donor; and 0.4 +/- 0.05 and 1.1 +/- 0.04 in cells from normal children (9-13 yr). For two cell strains from young adults, ILA50 and ILA95 were 0.30 +/- 0.02 and 1.0 +/- 0.3 ng eq/ml at 30% of their in vitro lifespan completed (%LC) and these values increased at rates of 0.005 ng eq/m...Continue Reading

References

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Citations

Mar 1, 1997·Medicinal Research Reviews·J Pallarés-TrujilloJ M Argilés
Nov 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S I Rattan, A Derventzi
Jul 1, 1986·Mechanisms of Ageing and Development·B M Stanulis-Praeger, B A Gilchrest
Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger
Mar 1, 1991·Mutation Research·A Derventzi, S I Rattan
Jun 18, 2002·Ageing Research Reviews·Vinod K Srivastava, David L Busbee
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S GoldsteinR C Baxter
Nov 15, 2003·Journal of Anti-aging Medicine·Michael Fossel
Jul 1, 1984·Clinics in Dermatology·B A Gilchrest
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Josep M ArgilésFrancisco J López-Soriano
May 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J F Dice
May 1, 1988·The Journal of Investigative Dermatology·B M Stanulis-PraegerB A Gilchrest

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