PMID: 2497461May 1, 1989Paper

Decreased susceptibility of a 70-kDa protein to cathepsin L after phosphorylation by protein kinase C

Proceedings of the National Academy of Sciences of the United States of America
S LaumasE Racker

Abstract

A 70-kDa protein is phosphorylated in cell-free preparations from rat or mouse fibroblasts by an endogenous protein kinase. This protein is immunologically related to a group of 68-kDa to 87-kDa proteins described in the literature as substrates for protein kinase C (PK-C). Although the phosphorylation of the 70-kDa protein by isolated plasma membranes takes place in the presence of EGTA, we conclude that the reaction is catalyzed by PK-C based on its inhibition by staurosporin. As shown previously, pure PK-C phosphorylates a synthetic random polymer of arginine and serine in the absence of Ca2+ and lipids, a reaction markedly stimulated by an endogenous unidentified activator of PK-C. When the 70-kDa protein from normal fibroblasts was exposed to the cytosol of chemically or ras-transformed fibroblasts, it disappeared as measured by phosphorylation by added PK-C. Cytosol of normal fibroblasts was much less effective (ca. 20%). Cathepsin L purified from rat kidney or from the medium of transformed cells had an effect similar to that of the cytosol of transformed cells. When the 70-kDa protein was phosphorylated by PK-C prior to exposure to cathepsin L or to the cytosol of transformed cells, there was a marked protection of the ...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M M Gottesman
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PrywesR G Roeder
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M Abdel-GhanyE Racker
Feb 29, 1988·Biochemical and Biophysical Research Communications·Z ZhangA Stracher
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
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Jul 30, 1981·Biochemical and Biophysical Research Communications·M Nilsen-HamiltonS L Massoglia

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Citations

Jan 1, 1996·Clinical & Experimental Metastasis·M SivaparvathiJ S Rao
Feb 7, 2002·The Biochemical Journal·Anna ArbuzovaGuy Vergères
May 3, 2008·The Journal of Biological Chemistry·Ghada Abboud-JarrousIsrael Vlodavsky
Jun 8, 1999·The Journal of Biological Chemistry·S CorradinG Vergères
Aug 28, 1999·The Journal of Biological Chemistry·S CorradinG Vergères

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