Decreased Susceptibility to Macrolide-Lincosamide in Mycoplasma synoviae Is Associated with Mutations in 23S Ribosomal RNA

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Inna LysnyanskySalvatore Catania

Abstract

The mechanism responsible for acquired decreased susceptibility to macrolides (14-membered erythromycin [Ery], 16-membered tylosin [Ty] and tilmicosin [Tm]) and to lincosamides (lincomycin [Ln]) was investigated in Mycoplasma synoviae, a pathogen that causes respiratory infections and synovitis in chicken and turkey. Sequence analysis of domains II and V of the two 23S rRNA alleles and ribosomal proteins L4 and L22 was performed on 49 M. synoviae isolates, M. synoviae type strain WVU1853, and reference strain FMT showing minimal inhibitory concentrations (MICs) to Ty (≤ 0.015 to 2 μg/ml), Tm (0.03 to ≥ 8 μg/ml), and Ln (0.125 to 8 μg/ml); MICs to Ery ranged from 32 to ≥ 128 μg/ml. Our results showed that the nucleotide substitution G748A (Escherichia coli numbering) in domain II of one or both 23S rRNA alleles may account for a slight increase in MICs to Ty and Tm (up to 0.5 and 2 μg/ml, respectively). No correlation between the presence of G748A and decreased susceptibility to Ln was found. However, the presence of the point mutations A2058G or A2059G in domain V of one or both alleles of the 23S rRNAs was correlated with a more significant decrease in susceptibility to Ty (1-2 μg/ml), Tm (≥ 8 μg/ml), and Ln (≥ 8 μg/ml). All M...Continue Reading

References

Dec 1, 1996·Revue Scientifique Et Technique·L Stipkovits, I Kempf
Sep 26, 1997·Antimicrobial Agents and Chemotherapy·P C HannanM Stegemann
Apr 5, 2000·The Journal of Antimicrobial Chemotherapy·P M FurneriL S Roccasalva
Dec 20, 2000·Antimicrobial Agents and Chemotherapy·B Vester, S Douthwaite
Jan 18, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Roland Leclercq
Aug 2, 2002·Molecular Cell·Jeffrey L HansenThomas A Steitz
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mingfu Liu, Stephen Douthwaite
Dec 6, 2003·The Journal of Antimicrobial Chemotherapy·Ian PhillipsJohn Waddell
Sep 6, 2005·The Journal of Veterinary Medical Science·Hideki KobayashiKoshi Yamamoto
Oct 6, 2005·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Tim StakenborgFreddy Haesebrouck
Aug 19, 2008·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Meng DongyaWang Lu
Nov 8, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jørgen S JensenRyoichi Hamasuna
Jan 22, 2009·Avian Pathology : Journal of the W.V.P.A·A FeberweeW J M Landman
Mar 1, 2011·International Journal of Antimicrobial Agents·Li XiaoKen B Waites
Apr 30, 2011·Future Microbiology·Cécile BébéarOlivia Peuchant
Aug 30, 2011·The Veterinary Record·B W StrugnellR M Irvine
Aug 11, 2012·The Journal of Antimicrobial Chemotherapy·S GovenderL J Chalkley
Apr 25, 2013·Antimicrobial Agents and Chemotherapy·I LysnyanskyS Levisohn
Jan 8, 2014·Veterinary Microbiology·Uri LernerInna Lysnyansky

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Citations

Feb 9, 2016·Canadian Journal of Microbiology·Muhammad SulemanJose Perez-Casal
Nov 19, 2017·BMC Veterinary Research·Zsuzsa KreizingerMiklós Gyuranecz
Sep 2, 2018·Applied and Environmental Microbiology·Amanda BeylefeldCelia Abolnik
Dec 3, 2020·International Journal of Antimicrobial Agents·Olga A ChernovaRustam I Aminov
Jul 14, 2018·Microbiology Spectrum·Anne V Gautier-Bouchardon

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Methods Mentioned

BETA
PCRs
PCR

Software Mentioned

DNASTAR

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