Decreasing arsenic accumulation in rice by overexpressing OsNIP1;1 and OsNIP3;3 through disrupting arsenite radial transport in roots

The New Phytologist
Sheng-Kai SunFang-Jie Zhao

Abstract

Rice is a major dietary source of the toxic metalloid arsenic. Reducing arsenic accumulation in rice grain is important for food safety. We generated transgenic rice overexpressing two aquaporin genes, OsNIP1;1 and OsNIP3;3, under the control of a maize ubiquitin promoter or the rice OsLsi1 promoter, and tested the effect on arsenite uptake and translocation. OsNIP1;1 and OsNIP3;3 were highly permeable to arsenite in Xenopus oocyte assays. Both transporters were localized at the plasma membrane. Knockout of either gene had little effect on arsenite uptake or translocation. Overexpression of OsNIP1;1 or OsNIP3;3 in rice did not affect arsenite uptake but decreased root-to-shoot translocation of arsenite and shoot arsenic concentration markedly. The overexpressed OsNIP1;1 and OsNIP3;3 proteins were localized in all root cells without polarity. Expression of OsNIP1;1 driven by the OsLsi1 promoter produced similar effects. When grown in two arsenic-contaminated paddy soils, overexpressing lines contained significantly lower arsenic concentration in rice grain than the wild-type without compromising plant growth or the accumulation of essential nutrients. Overexpression of OsNIP1;1 or OsNIP3;3 provides a route for arsenite to leak o...Continue Reading

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Citations

Aug 14, 2019·Journal of Experimental Botany·Stephan Clemens
Dec 28, 2019·The New Phytologist·Jie ChenFang-Jie Zhao
Jun 26, 2020·Journal of Experimental Botany·Zhong TangFang-Jie Zhao
Jul 16, 2020·Plant, Cell & Environment·Jia-Dong ChangFang-Jie Zhao
Jun 17, 2020·Journal of Experimental Botany·Jia-Dong ChangFang-Jie Zhao
Aug 15, 2020·Frontiers in Genetics·Priyanka DasArun Lahiri Majumder
Jan 25, 2020·Plants·Tayebeh Abedi, Amin Mojiri
Jan 9, 2021·Journal of Hazardous Materials·Amos Musyoki MawiaPeisong Hu
Oct 27, 2020·Plant Direct·Lauren WhittIvan Baxter
Jan 17, 2021·Journal of Hazardous Materials·Sanskriti VatsRupesh Deshmukh
Nov 15, 2020·Journal of Hazardous Materials·Fenglin DengZhong-Hua Chen
Feb 23, 2020·Plant Physiology and Biochemistry : PPB·Roshan Kumar SinghManoj Prasad
Mar 1, 2021·Environmental Pollution·Ashish Kumar SrivastavaPenna Suprasanna
Mar 4, 2021·Nature Communications·Sheng-Kai SunFang-Jie Zhao
Jun 21, 2021·Chemosphere·Aditi Shreeya Bali, Gagan Preet Singh Sidhu
Jul 21, 2021·International Journal of Molecular Sciences·Sajad AliHanhong Bae
Aug 25, 2021·The Science of the Total Environment·Debojyoti MoulickSubhas Chandra Santra
Oct 9, 2021·Plant, Cell & Environment·Dorina Podar, Frans J M Maathuis
Nov 14, 2021·Biological Trace Element Research·Vívian Ebeling VianaCamila Pegoraro

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