Decreasing miRNA sequencing bias using a single adapter and circularization approach

Genome Biology
Sergio Barberán-SolerSergei A Kazakov

Abstract

The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.

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Citations

Sep 4, 2018·Genome Biology·Sergio Barberán-SolerSergei A Kazakov
Nov 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tatsuo Adachi, Yoshikazu Nakamura
Sep 19, 2020·Journal of Extracellular Vesicles·S Eréndira Avendaño-Vázquez, C Fabián Flores-Jasso
Oct 23, 2019·Molecular Aspects of Medicine·Lukas ValihrachMikael Kubista
Feb 5, 2021·Chemical Society Reviews·Thomas JetValérie Taly
Feb 16, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chiara De SantiCatherine M Greene
Jun 3, 2021·Diagnostics·Sarka BenesovaLukas Valihrach
Oct 15, 2021·Cardiovascular Research·Maarten VanhaverbekeUNKNOWN EU-CardioRNA COST Action CA17129

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

RealSeq®
NEBNext®
AC
miRXplore
Bowtie2
QIAseq
RealSeq
TruSeq®
SMARTer

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